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PMID: 2304549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro replication through nucleosomes without histone displacement.

Nature ·Vol. 343 ·No. 6260 ·1990-02-22 ·Pages 719-26

Bonne-Andrea C, Wong ML, Alberts BM

Abstract

A well-characterized set of proteins encoded by bacteriophage T4 replicates DNA in vitro and generates replication forks that can pass nucleosomes. The histone octamers remain associated with newly replicated DNA even in the presence of excess DNA competitor, and intact nucleosomes re-form on the two daughter DNA helices. It is concluded that nucleosomes are designed to open up transiently to allow the passage of a replication fork without histone displacement.

MeSH Terms

DNA Replication DNA, Circular/genetics DNA, Viral/biosynthesis,metabolism Histones/metabolism Macromolecular Substances Microscopy, Electron Nucleosomes/metabolism,ultrastructure T-Phages/genetics,physiology Templates, Genetic Virus Replication

Chemicals

DNA, Circular DNA, Viral Histones Macromolecular Substances Nucleosomes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bonne-Andrea C

Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.

Wong M L

Alberts B M

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/343719a0

Published

1990-02-22

Pages

719-26

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/343719a0 View journal details

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