Purification of pig synovial collagenase to high specific activity.

The Biochemical journal ·Vol. 183 ·No. 3 ·1979-12-01 ·Pages 647-56

Cawston TE, Tyler JA

Abstract

1. Pig synovium in tissue culture secretes a specific collagenase in a latent form. 2. The latent enzyme was concentrated by (NH4)2SO4 precipitation and activated with 4-aminophenylmercuric acetate, and the active enzyme was purified by chromatography on Ultrogel AcA44, DEAE-cellulose, heparin-Sepharose and a zinc-chelate medium to a specific activity of 53 400 units/mg. of protein. 3. The enzyme was shown to be essentially homogeneous by polyacrylamide-gel electrophoresis. 4. The purified collagenase digested collagen to give the characteristic three-quarter and one-quarter pieces.

MeSH Terms

Animals Chromatography, Liquid Collagen/metabolism Culture Techniques Electrophoresis, Polyacrylamide Gel Enzyme Activation/drug effects Methods Microbial Collagenase/isolation & purification,metabolism Phenylmercuric Acetate/analogs & derivatives,pharmacology Swine Synovial Membrane/enzymology

Chemicals

Collagen Microbial Collagenase Phenylmercuric Acetate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cawston T E

Tyler J A

References (29)

29 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1830647

Published

1979-12-01

Pages

647-56

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161646

Subset

External Links

PubMed source DOI: 10.1042/bj1830647 PMC Full Text View journal details

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Purification of pig synovial collagenase to high specific activity.

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