Utilization of mucin by oral Streptococcus species.

Antonie van Leeuwenhoek ·Vol. 57 ·No. 3 ·1990-04-00 ·Pages 165-72

van der Hoeven JS, van den Kieboom CW, Camp PJ

Abstract

The ability of oral Streptococcus strains to utilize oligosaccharide chains in mucin as a source of carbohydrate was studied in batch cultures. Pig gastric mucin, as a substitute of human salivary mucin, was added to chemically defined medium containing no other carbohydrates. Strains of S. mitior attained the highest cell density, while mutans streptococci: S. mutans, S. sobrinus, S. rattus, grew very little in the medium with mucin. S. mitis, S. sanguis, and S. milleri in decreasing order, showed intermediate growth. Mucin breakdown as measured by sugar analyses indicated that oligosaccharide chains were only partially degraded. Every strain produced one or more exoglycosidases potentially involved in hydrolysis of oligosaccharide. The enzyme activities occurred mainly associated with the cells, and very little activity was found in the culture fluids. The relationships between glycosidase activities and growth, or mucin degradation were not always clear.

MeSH Terms

Animals DNA, Bacterial/analysis Fermentation Hydrogen-Ion Concentration Mucins/metabolism Rats Streptococcus/growth & development,metabolism Swine

Chemicals

DNA, Bacterial Mucins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

van der Hoeven J S

Laboratory of Oral Microbiology, University of Nijmegen, The Netherlands.

van den Kieboom C W

Camp P J

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25 references, click to expand

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Article Info

Journal

Antonie van Leeuwenhoek

Abbr.

Antonie Van Leeuwenhoek

ISSN

0003-6072

DOI

10.1007/BF00403951

Published

1990-04-00

Pages

165-72

Language

English

Region

Netherlands

NLM ID

0372625

Subset

External Links

PubMed source DOI: 10.1007/BF00403951 View journal details

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Utilization of mucin by oral Streptococcus species.

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