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PMID: 23303778 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Replication-independent nucleosome exchange is enhanced by local and specific acetylation of histone H4.

Nucleic acids research ·Vol. 41 ·No. 4 ·2013-02-01 ·Pages 2228-38

Elliott GO, Murphy KJ, Hayes JJ, Thiriet C

Abstract

We used a novel single-cell strategy to examine the fate of histones during G(2)-phase. Consistent with previous results, we find that in G(2)-phase, the majority of nuclear histones are assembled into chromatin, whereas a small fraction comprises an unassembled pool. Small increases in the amount of histones within the free pool affect the extent of exchange, suggesting that the free pool is in dynamic equilibrium with chromatin proteins. Unexpectedly, acetylated H4 is preferentially partitioned to the unassembled pool. Although an increase in global histone acetylation did not affect overall nucleosome dynamics, an H4 containing lysine to glutamine substitutions as mimics of acetylation significantly increased the rate of exchange, but did not affect the acetylation state of neighbouring nucleosomes. Interestingly, transcribed regions are particularly predisposed to exchange on incorporation of H4 acetylation mimics compared with surrounding regions. Our results support a model whereby histone acetylation on K8 and K16 specifically marks nucleosomes for eviction, with histones being rapidly deacetylated on reassembly.

MeSH Terms
Acetylation Cell Nucleus/metabolism Chromatin/metabolism Chromatin Assembly and Disassembly DNA Replication G2 Phase Histones/metabolism Nucleosomes/metabolism Physarum polycephalum/metabolism Single-Cell Analysis
Chemicals
Chromatin Histones Nucleosomes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Elliott Giles O
UFIP (FRE-CNRS 3478), Université de Nantes, 2 rue de la Houssinière, 44322 Nantes Cedex 3, France.
Murphy Kevin J
Hayes Jeffrey J
Thiriet Christophe
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gks1451
Published
2013-02-01
Epub
2013-00-08
Pages
2228-38
Language
English
Region
England
NLM ID
0411011
PMCID
PMC3575802
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052426 · United States
NIGMS NIH HHS · T32 GM068411 · United States
NIGMS NIH HHS · GM52426 · United States
External Links
PubMed source DOI: 10.1093/nar/gks1451 PMC Full Text View journal details
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