Home LiteratureArticle Details
PMID: 2332472 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Prevalence of seroreactors to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae in swine herds.

Journal of clinical microbiology ·Vol. 28 ·No. 4 ·1990-04-00 ·Pages 789-91

Devenish J, Rosendal S, Bossé JT, Wilkie BN, Johnson R

Abstract

A rabbit homologous polyclonal antiserum to the 104-kilodalton hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was specifically produced and used in an antigen capture enzyme-linked immunosorbent assay (ELISA) to detect swine serum antibodies to this potentially important virulence factor. Sera from pigs experimentally infected with the most common disease-producing serotypes (serotypes 1, 2, 5, and 7) of A. pleuropneumoniae produced positive results in this ELISA. Of 144 serum samples collected from 10 herds free of pleuropneumonia and 155 serum samples from 11 herds with a history of the disease, 68 (47%) and 148 (95%), respectively, were found positive by the ELISA. In addition, pigs naturally infected with Actinobacillus suis produced antibodies which seroreacted in this ELISA. The results indicated that a high proportion of swine have antibodies seroreactive with the 104-kilodalton hemolysin produced by A. pleuropneumoniae.

MeSH Terms
Actinobacillus/immunology Animals Antibodies, Bacterial/analysis Enzyme-Linked Immunosorbent Assay Hemolysin Proteins/immunology Rabbits Swine/immunology,microbiology
Chemicals
Antibodies, Bacterial Hemolysin Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Devenish J
Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada.
Rosendal S
Bossé J T
Wilkie B N
Johnson R
References (18)
18 references, click to expand
  1. Morphological and biochemical comparison of virulent and avirulent isolates of Haemophilus pleuropneumoniae serotype 5.
    Infect Immun. 1986 Feb;51(2):419-24 PMID: 3943895
  2. Haemophilus pleuropneumoniae serotypes--cross protection experiments.
    Nord Vet Med. 1984 Jul-Aug;36(7-8):221-34 PMID: 6493974
  3. Acute inflammatory effects of intratracheally instilled Escherichia coli endotoxin and sonicated suspension of Haemophilus pleuropneumoniae in swine.
    Can J Vet Res. 1986 Oct;50(4):526-31 PMID: 3539296
  4. Enzyme immunoassay: observations on aspects of quality control.
    Vet Immunol Immunopathol. 1987 Dec;17(1-4):441-52 PMID: 3324469
  5. Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test.
    J Clin Microbiol. 1983 May;17(5):787-90 PMID: 6190838
  6. Evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of Haemophilus (Actinobacillus) pleuropneumoniae.
    Am J Vet Res. 1988 Jul;49(7):1053-8 PMID: 3421527
  7. Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli.
    Infect Immun. 1989 Oct;57(10):3210-3 PMID: 2674017
  8. Analysis of major antigens of Haemophilus (Actinobacillus) pleuropneumoniae and related organisms.
    Infect Immun. 1987 Jul;55(7):1626-34 PMID: 3298061
  9. Isolation and biological characterization of two lipopolysaccharides and a capsular-enriched polysaccharide preparation from Haemophilus pleuropneumoniae.
    Am J Vet Res. 1986 Jul;47(7):1433-41 PMID: 3740612
  10. Evaluation of different antigens in the complement-fixation test for diagnosis of Haemophilus pleuropneumoniae (parahaemolyticus) infections in swine.
    Am J Vet Res. 1979 Nov;40(11):1564-7 PMID: 525874
  11. Comparative virulence of porcine Haemophilus bacteria.
    Can J Comp Med. 1985 Jan;49(1):68-74 PMID: 3986682
  12. An enzyme-linked immunosorbent assay, using an EDTA-extracted antigen for the serology of Haemophilus pleuropneumoniae.
    Am J Vet Res. 1981 Dec;42(12):2139-42 PMID: 6176151
  13. Regulation of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca2+.
    Infect Immun. 1988 Oct;56(10):2570-5 PMID: 3417350
  14. The haemolysins of Escherichia coli.
    J Pathol Bacteriol. 1963 Jan;85:197-211 PMID: 13989441
  15. The Use of an Enzyme-linked Immunosorbent Assay for Diagnosis of Actinobacillus pleuropneumoniae Infection in Pigs.
    Can Vet J. 1988 Jul;29(7):583-5 PMID: 17423083
  16. Role of haemophilus pleuropneumoniae lipopolysaccharide endotoxin in the pathogenesis of porcine Haemophilus pleuropneumonia.
    Am J Vet Res. 1987 May;48(5):768-73 PMID: 3592376
  17. Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1.
    Can J Vet Res. 1989 Apr;53(2):251-4 PMID: 2713790
  18. Ontario. Actinobacillus suis septicemia mimicking erysipelas in sows.
    Can Vet J. 1988 Jul;29(7):595 PMID: 17423089
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.28.4.789-791.1990
Published
1990-04-00
Pages
789-91
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267796
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.28.4.789-791.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2