Home LiteratureArticle Details
PMID: 2333305 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and characterization of a HeLa nuclear protein that specifically binds to the trans-activation-response (TAR) element of human immunodeficiency virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3624-8

Marciniak RA, Garcia-Blanco MA, Sharp PA

Abstract

Human immunodeficiency virus type 1 RNAs contain a sequence, trans-activation-response (TAR) element, which is required for tat protein-mediated trans-activation of viral gene expression. We have identified a nuclear protein from extracts of HeLa cells that binds to the TAR element RNA in a sequence-specific manner. The binding of this 68-kDa polypeptide was detected by UV cross-linking proteins to TAR element RNA transcribed in vitro. Competition experiments were performed by using a partially purified preparation of the protein to quantify the relative binding affinities of TAR element RNA mutants. The binding affinity of the TAR mutants paralleled the reported ability of those mutants to support tat trans-activation in vivo. We propose that this cellular protein moderates TAR activity in vivo.

MeSH Terms
Base Sequence Binding Sites Binding, Competitive HIV/genetics HeLa Cells/metabolism Humans Kinetics Molecular Sequence Data Nuclear Proteins/isolation & purification,metabolism,radiation effects Nucleic Acid Conformation Oligonucleotide Probes Plasmids RNA, Viral/genetics,metabolism Restriction Mapping Transcriptional Activation Ultraviolet Rays
Chemicals
Nuclear Proteins Oligonucleotide Probes RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marciniak R A
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge.
Garcia-Blanco M A
Sharp P A
References (33)
33 references, click to expand
  1. Activation of interferon-regulated, dsRNA-dependent enzymes by human immunodeficiency virus-1 leader RNA.
    Nucleic Acids Res. 1989 Feb 11;17(3):969-78 PMID: 2922280
  2. Activation of double-stranded RNA-dependent kinase (dsl) by the TAR region of HIV-1 mRNA: a novel translational control mechanism.
    Cell. 1989 Jan 27;56(2):303-12 PMID: 2536299
  3. Specific binding of a HeLa cell nuclear protein to RNA sequences in the human immunodeficiency virus transactivating region.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):4858-62 PMID: 2544877
  4. Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6925-9 PMID: 2476805
  5. HIV TAR: an RNA enhancer?
    Cell. 1989 Oct 20;59(2):229-30 PMID: 2680105
  6. Tat trans-activates the human immunodeficiency virus through a nascent RNA target.
    Cell. 1989 Oct 20;59(2):273-82 PMID: 2478293
  7. Identification of cellular proteins that bind to the human immunodeficiency virus type 1 trans-activation-responsive TAR element RNA.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):7828-32 PMID: 2510154
  8. trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis.
    J Virol. 1989 Dec;63(12):5501-4 PMID: 2479775
  9. Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
    Genes Dev. 1989 Dec;3(12A):1874-86 PMID: 2533575
  10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  11. Photo-induced joining of a transfer RNA with its cognate aminoacyl-transfer RNA synthetase.
    J Mol Biol. 1974 Apr 25;84(4):503-13 PMID: 4840999
  12. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  13. Some simple computational methods to improve the folding of large RNAs.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):45-52 PMID: 6198623
  14. Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.
    Cell. 1984 Jun;37(2):415-27 PMID: 6722880
  15. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  16. Trans-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat.
    Science. 1985 Jan 11;227(4683):171-3 PMID: 2981427
  17. The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
    Cell. 1985 Jul;41(3):813-23 PMID: 2988790
  18. Post-transcriptional regulation accounts for the trans-activation of the human T-lymphotropic virus type III.
    Nature. 1986 Feb 13-19;319(6054):555-9 PMID: 3003584
  19. The trans-activator gene of the human T cell lymphotropic virus type III is required for replication.
    Cell. 1986 Mar 28;44(6):941-7 PMID: 2420471
  20. The trans-activator gene of HTLV-III is essential for virus replication.
    Nature. 1986 Mar 27-Apr 2;320(6060):367-71 PMID: 3007995
  21. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
    J Virol. 1986 Aug;59(2):284-91 PMID: 3016298
  22. Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism.
    Cell. 1986 Sep 26;46(7):973-82 PMID: 3530501
  23. Elevated levels of mRNA can account for the trans-activation of human immunodeficiency virus.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9734-8 PMID: 3025848
  24. Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein.
    Cell. 1987 Feb 27;48(4):691-701 PMID: 3643816
  25. Trans-activation of human immunodeficiency virus gene expression is mediated by nuclear events.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6364-8 PMID: 3476953
  26. Mutational analysis of the trans-activation-responsive region of the human immunodeficiency virus type I long terminal repeat.
    J Virol. 1988 Mar;62(3):673-9 PMID: 2828663
  27. A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motif.
    Cell. 1988 Jan 29;52(2):221-8 PMID: 2830023
  28. Detection of activated double-stranded RNA-dependent protein kinase in 3T3-F442A cells.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1427-31 PMID: 2449691
  29. Tat protein from human immunodeficiency virus forms a metal-linked dimer.
    Science. 1988 Apr 1;240(4848):70-3 PMID: 2832944
  30. HIV-1 tat trans-activation requires the loop sequence within tar.
    Nature. 1988 Jul 14;334(6178):165-7 PMID: 3386755
  31. A discrete element 3' of human immunodeficiency virus 1 (HIV-1) and HIV-2 mRNA initiation sites mediates transcriptional activation by an HIV trans activator.
    Mol Cell Biol. 1988 Jun;8(6):2555-61 PMID: 2841583
  32. Regulation of HIV and HTLV gene expression.
    Genes Dev. 1988 Sep;2(9):1055-62 PMID: 2847958
  33. Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation.
    EMBO J. 1989 Mar;8(3):765-78 PMID: 2721501
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3624
Published
1990-05-00
Pages
3624-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53954
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.9.3624 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2