Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.

Archives of microbiology ·Vol. 153 ·No. 3 ·1990-00-00 ·Pages 235-40

Simonet P, Normand P, Moiroud A, Bardin R

Abstract

A set of oligonucleotides has been developed to study the competitivity of two Frankia strains in the nodulation of the roots of two host plant species: Alnus glutinosa and Alnus incana. Two 20 mer-oligonucleotides, complementary to highly conserved sequences inside the nifH gene, were used as primers for the polymerase chain reaction (PCR) system in order to amplify microsymbiont DNA extracted from actinorhizae. PCR products were analyzed using two strain-specific 15-mer oligonucleotides identified in the amplified region. Hybridization data indicate that strain ACoN24d is more competitive than strain ArI3 in the nodulation of both hosts.

MeSH Terms

Actinomycetales/genetics,isolation & purification Base Sequence Blotting, Southern DNA, Bacterial/analysis,genetics Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plants Polymerase Chain Reaction Species Specificity

Chemicals

DNA, Bacterial Oligonucleotide Probes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Simonet P

Laboratoire de Biologie des Sols, U.A. CNRS 697, Université Lyon 1, Villeurbanne, France.

Normand P

Moiroud A

Bardin R

References (10)

10 references, click to expand

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Article Info

Journal

Archives of microbiology

Abbr.

Arch Microbiol

ISSN

0302-8933

DOI

10.1007/BF00249074

Published

1990-00-00

Pages

235-40

Language

English

Region

Germany

NLM ID

0410427

Subset

External Links

PubMed source DOI: 10.1007/BF00249074 View journal details

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Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.

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