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PMID: 2335830 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In vitro-synthesized hepatitis delta virus RNA initiates genome replication in cultured cells.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 3104-7

Glenn JS, Taylor JM, White JM

Abstract

Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated products identified them as faithful copies of the hepatitis delta virus genome found in virions.

MeSH Terms
Animals Antigens, Viral/analysis Base Sequence Blotting, Northern Blotting, Western Cell Line Cells, Cultured DNA Replication DNA, Viral/genetics Fibroblasts/metabolism Genes, Viral Hepatitis Delta Virus/genetics Liposomes Mice Molecular Sequence Data RNA, Viral/administration & dosage,chemical synthesis,genetics
Chemicals
Antigens, Viral DNA, Viral Liposomes RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Glenn J S
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0450.
Taylor J M
White J M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.3104-3107.1990
Published
1990-06-00
Pages
3104-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249503
Subset
IM
Grants
NIAID NIH HHS · AI22470 · United States
NIAID NIH HHS · AI26522 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.6.3104-3107.1990 PMC Full Text View journal details
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