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PMID: 23370718 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root.

Plant physiology ·Vol. 161 ·No. 2 ·2013-02-00 ·Pages 931-41

Antoni R, Gonzalez-Guzman M, Rodriguez L, Peirats-Llobet M, Pizzio GA, Fernandez MA, De Winne N, De Jaeger G, Dietrich D, Bennett MJ, Rodriguez PL

Abstract

Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis (Arabidopsis thaliana) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA-mediated root growth inhibition. This effect was due to the lack of PYL8-mediated inhibition of several clade A phosphatases type 2C (PP2Cs), since PYL8 interacted in vivo with at least five PP2Cs, namely HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-INSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABA-hypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA-insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA-dependent inhibition of PP2Cs by PYR/PYLs is required for the proper perception of a moisture gradient.

MeSH Terms
Abscisic Acid/metabolism,pharmacology Arabidopsis/drug effects,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Gene Expression Regulation, Plant/drug effects Germination/drug effects Immunoblotting Mass Spectrometry Mutation Phosphoprotein Phosphatases/genetics,metabolism Plant Growth Regulators/metabolism,pharmacology Plant Roots/drug effects,genetics,metabolism Plants, Genetically Modified Protein Binding Proteome/genetics,metabolism Seeds/drug effects,genetics,metabolism Signal Transduction Water/metabolism,pharmacology
Chemicals
Arabidopsis Proteins PYL8 protein, Arabidopsis Plant Growth Regulators Proteome Water Abscisic Acid ABI1 protein, Arabidopsis ABI2 protein, Arabidopsis AHG3 protein, Arabidopsis HAB1 protein, Arabidopsis HAB2 protein, Arabidopsis Phosphoprotein Phosphatases
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Antoni Regina
Instituto de Biologia Molecular y Celular de Plantas, Consejo Superior de Investigaciones Cientificas-Universidad Politecnica de Valencia, ES-46022 Valencia, Spain.
Gonzalez-Guzman Miguel
Rodriguez Lesia
Peirats-Llobet Marta
Pizzio Gaston A
Fernandez Maria A
De Winne Nancy
De Jaeger Geert
Dietrich Daniela
Bennett Malcom J
Rodriguez Pedro L
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.112.208678
Published
2013-02-00
Epub
2012-00-14
Pages
931-41
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3561030
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/D019613/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/G023972/1 · United Kingdom
External Links
PubMed source DOI: 10.1104/pp.112.208678 PMC Full Text View journal details
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