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PMID: 233898 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histocompatibility antigen-activated cytotoxic T lymphocytes. I. Estimates of the absolute frequency of killer cells generated in vitro.

The Journal of experimental medicine ·Vol. 145 ·No. 3 ·1977-03-01 ·Pages 500-7

Lindahl KF, Wilson DB

Abstract

A sensitive procedure employing limiting dilution of activated lymphocytes and 51Cr release from highly labeled target cells was used to derive minimal estimates of the absolute frequency of cytotoxic T lymphocytes (CTL) present in a population of mouse lymphocytes activated to alloantigens of the major histocompatibility complex in bulk mixed lymphocyte cultures. From this figure (0.7-1.2%), the maximal rate of target cell killing could be calculated to be approximately 4 targets/CTL/hour.

MeSH Terms
Animals Cytotoxicity, Immunologic Female Histocompatibility Antigens/immunology Killer Cells, Natural/immunology Lymphocyte Activation Lymphocyte Subsets Male Mice Mice, Inbred Strains T-Lymphocytes, Cytotoxic/immunology Tumor Cells, Cultured
Chemicals
Histocompatibility Antigens
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lindahl K F
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19174.
Wilson D B
References (9)
9 references, click to expand
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  9. QUANTITATIVE STUDIES ON THE BEHAVIOR OF SENSITIZED LYMPHOCYTES IN VITRO : I. RELATIONSHIP OF THE DEGREE OF DESTRUCTION OF HOMOLOGOUS TARGET CELLS TO THE NUMBER OF LYMPHOCYTES AND TO THE TIME OF CONTACT IN CULTURE AND CONSIDERATION OF THE EFFECTS OF ISOIMMUNE SERUM.
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.145.3.500
Published
1977-03-01
Pages
500-7
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180717
Subset
IM
Grants
NIAID NIH HHS · AI-10961 · United States
NCI NIH HHS · CA-15822 · United States
NCI NIH HHS · CA-18640 · United States
External Links
PubMed source DOI: 10.1084/jem.145.3.500 PMC Full Text View journal details
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