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PMID: 23399 Published · ppublish English Journal Article

Fractionation of cocksfoot (Dactylis glomerata) pollen by preparative isoelectric focussing.

Journal of immunological methods ·Vol. 19 ·No. 1 ·1978-00-00 ·Pages 61-7

Topping MD, Brighton WD, Stokell M, Patterson JM

Abstract

The technique of isoelectric focussing in polyacrylamide gel has been developed to a preparative mode. Using the improved technique cocksfoot (Dactylis glomerata) pollen has been split into many components, and the allergenic reactions of the preparations have been studied by passive transfer in animal skin and by RAST.

MeSH Terms
Animals Humans Hydrogen-Ion Concentration Isoelectric Focusing Passive Cutaneous Anaphylaxis Pollen/analysis Rats Reagins/analysis
Chemicals
Reagins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Topping M D
Brighton W D
Stokell M
Patterson J M
Article Info
Journal
Journal of immunological methods
Abbr.
J Immunol Methods
ISSN
0022-1759
DOI
10.1016/0022-1759(78)90008-x
Published
1978-00-00
Pages
61-7
Language
English
Region
Netherlands
NLM ID
1305440
Subset
IM
External Links
PubMed source DOI: 10.1016/0022-1759(78)90008-x View journal details
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