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PMID: 23454382 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Functional inhibition of UQCRB suppresses angiogenesis in zebrafish.

Biochemical and biophysical research communications ·Vol. 433 ·No. 4 ·2013-04-19 ·Pages 396-400

Cho YS, Jung HJ, Seok SH, Payumo AY, Chen JK, Kwon HJ

Abstract

As a subunit of mitochondrial complex III, UQCRB plays an important role in complex III stability, electron transport, and cellular oxygen sensing. Herein, we report UQCRB function regarding angiogenesis in vivo with the zebrafish (Danio rerio). UQCRB knockdown inhibited angiogenesis in zebrafish leading to the suppression of VEGF expression. Moreover, the UQCRB-targeting small molecule terpestacin also inhibited angiogenesis and VEGF levels in zebrafish, supporting the role of UQCRB in angiogenesis. Collectively, UQCRB loss of function by either genetic and pharmacological means inhibited angiogenesis, indicating that UQCRB plays a key role in this process and can be a prognostic marker of angiogenesis- and mitochondria-related diseases.

MeSH Terms
Amino Acid Sequence Animals Bridged Bicyclo Compounds/pharmacology Carrier Proteins/genetics,metabolism Dose-Response Relationship, Drug Embryo, Nonmammalian/drug effects,physiology Embryonic Development Gene Expression Regulation, Developmental Gene Knockdown Techniques Mitochondria/genetics,metabolism Molecular Sequence Data Morpholinos/pharmacology Neovascularization, Physiologic/genetics Prognosis Vascular Endothelial Growth Factor A/genetics,metabolism Zebrafish/genetics,physiology
Chemicals
Bridged Bicyclo Compounds Carrier Proteins Morpholinos Vascular Endothelial Growth Factor A ubiquinone-binding proteins terpestacin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cho Yoon Sun
Chemical Genomics National Research Laboratory, Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science & Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea.
Jung Hye Jin
Seok Seung Hyeok
Payumo Alexander Y
Chen James K
Kwon Ho Jeong
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Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
1090-2104
DOI
10.1016/j.bbrc.2013.02.082
Published
2013-04-19
Epub
2013-00-28
Pages
396-400
Language
English
Region
United States
NLM ID
0372516
PMCID
PMC3691074
Subset
IM
Grants
NICHD NIH HHS · DP1 HD075622 · United States
NCCDPHP CDC HHS · DP1HD075622 · United States
External Links
PubMed source DOI: 10.1016/j.bbrc.2013.02.082 PMC Full Text View journal details
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