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PMID: 23483801 Published · ppublish English Journal Article

GPER1/GPR30 activation improves neuronal survival following global cerebral ischemia induced by cardiac arrest in mice.

Translational stroke research ·Vol. 3 ·No. 4 ·2012-12-01 ·Pages 500-507

Kosaka Y, Quillinan N, Bond C, Traystman R, Hurn P, Herson P

Abstract

Female sex steroids, particularly estrogens, contribute to the sexually dimorphic response observed in cerebral ischemic outcome, with females being relatively protected compared to males. Using a mouse model of cardiac arrest and cardiopulmonary resuscitation (CA/CPR), we previously demonstrated that estrogen neuroprotection is mediated in part by the estrogen receptor β, with no involvement of estrogen receptor α. In this study we examined the neuroprotective effect of the novel estrogen receptor, G-protein coupled estrogen receptor 1 (GPER1/GPR30). Male mice administered the GPR30 agonist G1 exhibited significantly reduced neuronal injury in the hippocampal CA1 region and striatum. The magnitude of neuroprotection observed in G1 treated mice was indistinguishable from estrogen treated mice, implicating GPR30 in estrogen neuroprotection. Real-time quantitative RT-PCR indicates that G1 treatment increases expression of the neuroprotective ion channel, small conductance calcium-activated potassium channel 2. We conclude that GPR30 agonists show promise in reducing brain injury following global cerebral ischemia.

Keywords
Cardiac arrest G1 GPR30/GPER1 SK2 cerebral ischemia
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kosaka Y
Department of Anesthesiology and Perioperative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239.
Quillinan N
Bond Ct
Traystman Rj
Hurn Pd
Herson Ps
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Article Info
Journal
Translational stroke research
Abbr.
Transl Stroke Res
ISSN
1868-601X
DOI
10.1007/s12975-012-0211-8
Published
2012-12-01
Epub
2012-00-12
Pages
500-507
Language
English
Region
United States
NLM ID
101517297
PMCID
PMC3587667
Grants
NINR NIH HHS · R01 NR003521 · United States
NINDS NIH HHS · R01 NS046072 · United States
NINDS NIH HHS · R01 NS058792 · United States
External Links
PubMed source DOI: 10.1007/s12975-012-0211-8 PMC Full Text View journal details
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