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PMID: 2348833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing of two actin genes from Taenia solium (Cestoda).

Molecular and biochemical parasitology ·Vol. 40 ·No. 1 ·1990-04-00 ·Pages 87-93

Campos A, Bernard P, Fauconnier A, Landa A, Gómez E, Hernández R, Willms K, Laclette JP

Abstract

Genomic and cDNA actin clones were isolated from Taenia solium gene libraries. The actin genes are interrupted by intervening sequences. Protein coding regions of both genes predict the same amino acid sequence. cDNA sequence data indicate that at least one gene is expressed at the larval stage. Results from Northern and Western blots showed that T. solium expresses an actin transcript of about 1,400 bases and a protein of 45,000 Da.

MeSH Terms

Actins/genetics Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular DNA/genetics Gene Expression Gene Library Genes Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Taenia/genetics

Chemicals

Actins DNA

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Campos A

Department of Immunology, Universidad Nacional Autónoma de México, Mexico City.

Bernard P

Fauconnier A

Landa A

Gómez E

Hernández R

Willms K

Laclette J P

Article Info

Journal

Molecular and biochemical parasitology

Abbr.

Mol Biochem Parasitol

ISSN

0166-6851

DOI

10.1016/0166-6851(90)90082-w

Published

1990-04-00

Pages

87-93

Language

English

Region

Netherlands

NLM ID

8006324

Subset

Databases

GENBANK

M28996, M28997

External Links

PubMed source DOI: 10.1016/0166-6851(90)90082-w View journal details

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Cloning and sequencing of two actin genes from Taenia solium (Cestoda).

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