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PMID: 235125 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Evidence for adenosine diphosphate ribosylation of Ca2+, Mg2+-dependent endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 289-93

Yoshihara K, Tanigawa Y, Burzio L, Koide SS

Abstract

The molecular basis for the inhibition of the Ca2+,Mg2+-dependent endonuclease resulting from the formation of poly(adenosine diphosphate ribose) (ADP-Rib) was studies in a simplified system containing purified rat liver or bull semen endonuclease, purified rat liver poly(ADP-Rib) synthetase, [3H]NAD+, and DNA. Poly-(adp-rib) synthetase activity was stimulated when Ca2+, Mg2+-dependent endonuclease was added to the reaction mixture in place of histones, suggesting that the endonuclease can act as an acceptor for ADP-Rib. Evidence was presented to show that the ADP-Rib moiety of [3H]NAD+ was incorporated in the endonuclease fraction. The [3H]ADP-Rib bound to the endonuclease was in the form of monomers and oligomers and not long chain polymers. The present results suggest that the Ca2+,Mg2+-dependent endonuclease was ADP-ribosylated when the endonuclease was incubated with poly(ADP-Rib) synthetase and NAD+.

MeSH Terms

Adenosine Diphosphate/metabolism Animals Calcium Cattle Chromatin/metabolism Chromatography, Gel DNA/biosynthesis Endonucleases/metabolism Hydrogen-Ion Concentration Liver/metabolism Magnesium Male NAD/metabolism Rats Ribose/metabolism Semen/metabolism Tritium

Chemicals

Chromatin NAD Tritium Adenosine Diphosphate Ribose DNA Endonucleases Magnesium Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yoshihara K

Tanigawa Y

Burzio L

Koide S S

References (21)

21 references, click to expand

Synthesis of polyadenosine diphosphate ribose by isolated nuclei of swine aortic tissue.
Biochemistry. 1974 Mar 26;13(7):1327-30 PMID: 4362223
Poly(adenosine diphosphate ribose).
Prog Nucleic Acid Res Mol Biol. 1973;13:127-51 PMID: 4349570
In vitro effect of NAD on DNa synthesis in isolated nuclei from regenerating rat liver and novikoff hepatoma.
FEBS Lett. 1972 Jan 15;20(1):29-32 PMID: 11946374
Stimulation of DNA synthesis by adenosine diphosphoribosylation of HeLa nuclear proteins during the cell cycle.
Biochem Biophys Res Commun. 1973 May 1;52(1):43-50 PMID: 4576270
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Activity of polyadenosine diphosphoribose polymerase during the human cell cycle.
Biochem Biophys Res Commun. 1971 Jun 18;43(6):1266-73 PMID: 4328044
Inhibition of deoxyribonuclease in an extract of rat liver nuclei by poly(ADP-ribose).
Biochem Biophys Res Commun. 1974 Feb 27;56(4):1093-9 PMID: 4363639
Release of DNA polymerase from rat liver chromatin on incubation with NAD.
FEBS Lett. 1973 Sep 15;35(2):262-4 PMID: 4147598
Diphtheria toxin-dependent adenosine diphosphate ribosylation of aminoacyl transferase II and inhibition of protein synthesis.
J Biol Chem. 1968 Jun 25;243(12):3553-5 PMID: 4297784
The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.
Biochem Biophys Res Commun. 1973 May 15;52(2):475-81 PMID: 4575957
Inhibition of rat liver Ca2+, Mg2+-dependent endonuclease activity by nicotinamide adenine dinucleotide and poly (adenosine diphosphate ribose) synthetase.
Biochem Biophys Res Commun. 1974 Jul 24;59(2):658-65 PMID: 4368823
Poly ADP-ribose synthesis and DNA replication in synchronized mouse L-cells.
Biochem Biophys Res Commun. 1973 Aug 6;53(3):960-6 PMID: 4738721
Isolation and purification of calcium and magnesium dependent endonuclease from rat liver nuclei.
Biochem Biophys Res Commun. 1974 Feb 4;56(3):703-10 PMID: 4597066
A functional role of polyADPR in DNA synthesis.
Biochem Biophys Res Commun. 1970 Sep 10;40(5):1013-20 PMID: 4323263
Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD.
Biochem Biophys Res Commun. 1973 Jul 17;53(2):572-9 PMID: 4352070
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Chemical properties of elongation factor 2. Amino acid composition, NH 2 -terminal residue, and sulfhydryl reactivity.
J Biol Chem. 1972 Nov 10;247(21):7023-8 PMID: 5082139
The relationship between cell growth, macromolecular synthesis and poly ADP-ribose polymerase in lymphoid cells.
Exp Cell Res. 1974 Jan;83(1):63-72 PMID: 4360096
Complete dependency of poly)ADP-ribose) synthesis on DNA and its inhibition by actinomycin D.
Biochem Biophys Res Commun. 1972 Apr 14;47(1):119-25 PMID: 5027125
Studies on poly adenosine diphosphate-ribose. 8. A method for purification of poly adenosine diphosphate-ribose.
J Biol Chem. 1970 Mar 25;245(6):1327-30 PMID: 4315258
Enzymic adenosine diphosphate ribosylation of histone and poly adenosine diphosphate ribose synthesis in rat liver nuclei.
J Biol Chem. 1968 Jul 10;243(13):3765-7 PMID: 4298073

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.1.289

Published

1975-01-00

Pages

289-93

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432289

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.1.289 PMC Full Text View journal details

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Evidence for adenosine diphosphate ribosylation of Ca2+, Mg2+-dependent endonuclease.

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