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PMID: 23519396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate.

Journal of assisted reproduction and genetics ·Vol. 30 ·No. 4 ·2013-04-00 ·Pages 513-23

Bhilawadikar R, Zaveri K, Mukadam L, Naik S, Kamble K, Modi D, Hinduja I

Abstract

To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate. Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA. Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men. The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate. Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI.

MeSH Terms
Adult Asthenozoospermia/metabolism Calcium Channels/metabolism Cross-Sectional Studies Female Fertilization Fertilization in Vitro Humans Male Microtubule Proteins/metabolism Middle Aged Oligospermia/metabolism Pregnancy Pregnancy Rate Seminal Plasma Proteins/metabolism Sperm Injections, Intracytoplasmic Sperm Motility/physiology Spermatozoa/metabolism
Chemicals
CATSPER2 protein, human Calcium Channels Microtubule Proteins Seminal Plasma Proteins tektins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bhilawadikar Rashmi
Jaslok Hospital, 15, Dr G Deshmukh Marg, Mumbai - 26, India.
Zaveri Kusum
Mukadam Leena
Naik Shilpa
Kamble Ketan
Modi Deepak
Hinduja Indira
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Article Info
Journal
Journal of assisted reproduction and genetics
Abbr.
J Assist Reprod Genet
ISSN
1573-7330
DOI
10.1007/s10815-013-9972-6
Published
2013-04-00
Epub
2013-00-22
Pages
513-23
Language
English
Region
Netherlands
NLM ID
9206495
PMCID
PMC3644123
Subset
IM
External Links
PubMed source DOI: 10.1007/s10815-013-9972-6 PMC Full Text View journal details
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