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PMID: 2370845 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multiple proteins bind to the P2 promoter region of the zein gene pMS1 of maize.

Molecular & general genetics : MGG ·Vol. 221 ·No. 2 ·1990-04-00 ·Pages 164-70

Maier UG, Grasser KD, Haass MM, Feix G

Abstract

A 216 bp promoter fragment of the 19 kDa protein zein gene pMS1, containing the CCAAT and TATA boxes, was analysed by a variety of techniques for in vitro interactions with nuclear proteins from endosperm tissue. HMG proteins were found to form stable complexes with these A/T-rich promoter sequences and several specific DNA-binding proteins appear to be involved in the formation of DNA-protein complexes with this fragment. A 29 bp region spanning the two CCAAT boxes was protected from DNase I digestion in footprinting experiments.

MeSH Terms
Base Sequence Chromatography, Affinity DNA-Binding Proteins/metabolism Deoxyribonuclease I/metabolism Electrophoresis, Polyacrylamide Gel Genes, Plant High Mobility Group Proteins/metabolism Molecular Sequence Data Promoter Regions, Genetic Restriction Mapping Zea mays/genetics Zein/genetics
Chemicals
DNA-Binding Proteins High Mobility Group Proteins Zein Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Maier U G
Institute of Biology III, Freiburg, Federal Republic of Germany.
Grasser K D
Haass M M
Feix G
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00261716
Published
1990-04-00
Pages
164-70
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00261716
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