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PMID: 2371270 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional expression and subcellular localization of an anion exchanger cloned from choroid plexus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 14 ·1990-07-00 ·Pages 5278-82

Lindsey AE, Schneider K, Simmons DM, Baron R, Lee BS, Kopito RR

Abstract

We have isolated rat brain cDNA clones encoding AE2, a homologue of the erythrocyte anion exchanger, band 3 (AE1). Immunocytochemistry and in situ hybridization reveal that, in brain, AE2 expression is restricted to the basolateral membrane of the choroid plexus epithelium. Expression of a full-length mouse AE2 cDNA in COS-7 cells resulted in chloride- and bicarbonate-dependent alterations in intracellular pH, demonstrating that AE2 is a Cl/HCO3 exchanger. Cation replacement studies indicate that AE2-mediated exchange is independent of extracellular sodium. COS-7 cells expressing a mutant rat AE2 cDNA clone that lacks the cytoplasmic NH2-terminal 660 amino acids exhibit identical responses to cation and anion substitution. These results indicate that this domain does not play a significant role in either correct insertion of the transporter into the plasma membrane or anion exchange.

MeSH Terms
Amino Acid Sequence Animals Anion Exchange Protein 1, Erythrocyte/genetics Anion Transport Proteins Antiporters Cell Line Choroid Plexus/metabolism Cloning, Molecular DNA/genetics,isolation & purification Epithelium/metabolism Gene Expression Gene Library Hydrogen-Ion Concentration Membrane Proteins/analysis,genetics Mice Molecular Sequence Data Rats SLC4A Proteins Sequence Homology, Nucleic Acid Transfection
Chemicals
Anion Exchange Protein 1, Erythrocyte Anion Transport Proteins Antiporters Membrane Proteins SLC4A Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lindsey A E
Department of Biological Sciences, Stanford University, CA 94305-5020.
Schneider K
Simmons D M
Baron R
Lee B S
Kopito R R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.14.5278
Published
1990-07-00
Pages
5278-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54306
Subset
IM
Grants
NIGMS NIH HHS · GM38543 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.14.5278 PMC Full Text View journal details
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