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PMID: 237268 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Kinetics of steroid induction and deinduction of tyrosine aminotransferase synthesis in cultured hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2007-11

Steinberg RA, Levinson BB, Tomkins GM

Abstract

The specific rate of synthesis of tyrosine aminotransferase (EC 2.6.1.5; L-tyrosine:2-oxoglutarate aminotransferase) is used as a measure of the level of functional, cytoplasmic, tyrosine aminotransferase-specific mRNA in cultured rat hepatoma cells. An analysis of the kinetics of change in this rate after the addition or withdrawal of glucocorticosteroids sets an upper limit on the half-life of the enzyme-specific mRNA of 1-1.5 hr, whether or not steroid is present. The inactivation rate of the enzyme mRNA is independent of the growth condition of the cells, occuring equally rapidly in the presence or absence of serum or insulin, both of which induce tyrosine aminotransferase in these cells. The implications of these results for the mechanism of steroid induction are discussed.

MeSH Terms
Animals Blood Carcinoma, Hepatocellular/enzymology,metabolism Cattle Cells, Cultured Computers Dexamethasone/pharmacology Enzyme Induction/drug effects Insulin/pharmacology Kinetics Leucine/metabolism Liver Neoplasms Models, Chemical Precipitin Tests RNA, Messenger/metabolism Rats Tyrosine Transaminase/biosynthesis
Chemicals
Insulin RNA, Messenger Dexamethasone Tyrosine Transaminase Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Steinberg R A
Levinson B B
Tomkins G M
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2007
Published
1975-06-00
Pages
2007-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432681
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2007 PMC Full Text View journal details
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