Constructing DNA by polymerase recombination.

Nucleic acids research ·Vol. 18 ·No. 13 ·1990-07-11 ·Pages 3983-6

Yolov AA, Shabarova ZA

Abstract

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to be sufficient to provide further DNA joining also by use of PCR.

MeSH Terms

Base Sequence DNA, Recombinant/metabolism Gene Amplification Molecular Sequence Data Oligodeoxyribonucleotides/metabolism Plasmids Polymerase Chain Reaction

Chemicals

DNA, Recombinant Oligodeoxyribonucleotides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Yolov A A

Department of Chemistry, M.V. Lomonosov State University, Moscow, USSR.

Shabarova Z A

References (7)

7 references, click to expand

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Bioorg Khim. 1988 Aug;14(8):1034-9 PMID: 3219121
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.
Gene. 1989 Apr 15;77(1):61-8 PMID: 2744488
Genomic amplification with transcript sequencing.
Science. 1988 Jan 29;239(4839):491-4 PMID: 3340835
[Construction and expression of genes encoding short hybrid mRNA in Escherichia coli].
Dokl Akad Nauk SSSR. 1986 Mar-Apr;287(1):237-41 PMID: 2420548
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.13.3983

Published

1990-07-11

Pages

3983-6

Language

English

Region

England

NLM ID

0411011

PMCID

PMC331102

Subset

External Links

PubMed source DOI: 10.1093/nar/18.13.3983 PMC Full Text View journal details

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Constructing DNA by polymerase recombination.

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