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PMID: 2374739 Published · ppublish English Journal Article

Optimal conditions for directly sequencing double-stranded PCR products with sequenase.

Nucleic acids research ·Vol. 18 ·No. 13 ·1990-07-11 ·Pages 4028

Casanova JL, Pannetier C, Jaulin C, Kourilsky P

Abstract

暂无摘要

MeSH Terms
Base Sequence DNA DNA-Directed DNA Polymerase/metabolism Nucleic Acid Amplification Techniques Polymerase Chain Reaction/methods Temperature Time Factors
Chemicals
DNA bacteriophage T7 induced DNA polymerase DNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Casanova J L
Biologie Moléculaire du Gène, INSERM U277, CNRS UA535, Institut Pasteur, Paris, France.
Pannetier C
Jaulin C
Kourilsky P
References (8)
8 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA.
    Nucleic Acids Res. 1987 Jan 26;15(2):529-42 PMID: 2881260
  3. DNA typing from single hairs.
    Nature. 1988 Apr 7;332(6164):543-6 PMID: 3282169
  4. Improvement of PCR amplified DNA sequencing with the aid of detergents.
    Nucleic Acids Res. 1990 Mar 11;18(5):1309 PMID: 2320432
  5. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.
    Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40 PMID: 3200828
  6. An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.
    Nucleic Acids Res. 1989 Feb 11;17(3):1266 PMID: 2922271
  7. Direct sequencing from low-melt agarose with Sequenase.
    Nucleic Acids Res. 1989 Jul 25;17(14):5864 PMID: 2762168
  8. Diagnosis of alpha 1-antitrypsin deficiency by enzymatic amplification of human genomic DNA and direct sequencing of polymerase chain reaction products.
    Nucleic Acids Res. 1988 Sep 12;16(17):8233-43 PMID: 3262215
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.13.4028
Published
1990-07-11
Pages
4028
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331143
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.13.4028 PMC Full Text View journal details
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