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PMID: 237883 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase.

The Journal of biological chemistry ·Vol. 250 ·No. 1 ·1975-01-10 ·Pages 120-6

Hughes SH, Wahl GM, Capecchi MR

Abstract

Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations. The enzyme appears to be composed of 3 subunits of identical molecular weight (27,000 per subunit). The subunit molecular weight has also been determined by the analysis of radioactively labeled HGPR transferase immunoprecipitated from wild type and mutant (HGPR transferase) mouse tissue culture cell lines.

MeSH Terms
Animals Buffers Cell Line Centrifugation, Density Gradient Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Guanine Nucleotides Humans Hydrogen-Ion Concentration Hypoxanthines Inosine Nucleotides Isotope Labeling L Cells/enzymology Liver/enzymology Macromolecular Substances Mice Molecular Weight Pentosyltransferases/immunology,isolation & purification,metabolism Radioimmunoassay Sulfur Radioisotopes Tritium Ultrafiltration
Chemicals
Buffers Guanine Nucleotides Hypoxanthines Inosine Nucleotides Macromolecular Substances Sulfur Radioisotopes Tritium Pentosyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hughes S H
Wahl G M
Capecchi M R
Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1975-01-10
Pages
120-6
Language
English
Region
United States
NLM ID
2985121R
Subset
IM
External Links
PubMed source View journal details
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