Characterization of hepatic markers in human Wharton's Jelly-derived mesenchymal stem cells.

Toxicology in vitro : an international journal published in association with BIBRA ·Vol. 28 ·No. 1 ·2014-07-16

Buyl Karolien, De Kock Joery, Najar Mehdi, Lagneaux Laurence, Branson Steven, Rogiers Vera, Vanhaecke Tamara

Abstract

Stem cell technology could offer a unique tool to develop human-based in vitro liver models that are applicable for testing of potential liver toxicity early during drug development. In this context, recent research has indicated that human Wharton's Jelly-derived mesenchymal stem cells (hWJs) represent an interesting stem cell population to develop human hepatocyte-like cells. Here, an in-depth analysis of the expression of liver-specific transcription factors and other key hepatic markers in hWJs is evaluated at both the mRNA and protein level. Our results reveal that transcription factors that are mandatory to acquire and maintain an adult hepatic phenotype (HNF4A and HNF1A), as well as adult hepatic markers (ALB, CX32, CYP1A1, CYP1A2, CYP2B6 and CYP3A4) are not expressed in hWJs with the exception of K18. On the contrary, transcription factors involved in liver development (GATA4, GATA6, SOX9 and SOX17) and liver progenitor markers (DKK1, DPP4, DSG2, CX43 and K19) were found to be highly expressed in hWJs. These findings provide additional indication that hWJs could be a promising stem cell source to generate hepatocyte-like cells necessary for the development of a functional human-based in vitro liver model.

Keywords

4′ 6-diamidino-2-phenylindole ACTB AFP ALB Adult stem cells B2M CX CYP DAPI DKK DMEM-LG DPP DSG Dulbecco’s modified eagle medium-low glucose EGF FBS FGF FOX GAPDH GJ HEPs HMBS HNF Hepatocyte K KRT Liver development MSCs ND ONECUT PBS PFA SOX UBC Umbilical cord Wharton’s Jelly albumin beta-2-microglobulin beta-actin connexin cytochrome P450 desmoglein dickkopf dipeptidyl-peptidase epidermal growth factor fetal bovine serum fibroblast growth factor forkhead box gap junction glyceraldehyde 3-phosphate dehydrogenase hADSCs hASCs hBMSCs hSKPs hWJs hepatocyte nuclear factor human Wharton’s Jelly-derived mesenchymal stem cells human adipose tissue-derived stromal cells human adult stem cells human bone marrow-derived stromal cells human hepatocytes human skin-derived precursor cells hydroxy-methylbilane synthase keratin mesenchymal stem cells not significantly detected onecut homeobox paraformaldehyde phosphate buffered saline qPCR quantitative real-time reverse transcriptase polymerase chain reaction sex determining region Y-box ubiquitin C α-foetoprotein

Article Info

Journal

Toxicology in vitro : an international journal published in association with BIBRA

Abbr.

Toxicol In Vitro

ISSN

0887-2333

DOI

10.1016/j.tiv.2013.06.014

Published

2014-07-16

Indexed

2013-11-25

Updated

2016-11-25

Language

English

Country/Region

England

NLM ID

8712158

External Links

PubMed source DOI: 10.1016/j.tiv.2013.06.014 View journal details

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Characterization of hepatic markers in human Wharton's Jelly-derived mesenchymal stem cells.

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