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PMID: 23831726 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Apical domain polarization localizes actin-myosin activity to drive ratchet-like apical constriction.

Nature cell biology ·Vol. 15 ·No. 8 ·2013-08-00 ·Pages 926-36

Mason FM, Tworoger M, Martin AC

Abstract

Apical constriction promotes epithelia folding, which changes tissue architecture. During Drosophila gastrulation, mesoderm cells exhibit repeated contractile pulses that are stabilized such that cells apically constrict like a ratchet. The transcription factor Twist is required to stabilize cell shape. However, it is unknown how Twist spatially coordinates downstream signals to prevent cell relaxation. We find that during constriction, Rho-associated kinase (Rok) is polarized to the middle of the apical domain (medioapical cortex), separate from adherens junctions. Rok recruits or stabilizes medioapical myosin II (Myo-II), which contracts dynamic medioapical actin cables. The formin Diaphanous mediates apical actin assembly to suppress medioapical E-cadherin localization and form stable connections between the medioapical contractile network and adherens junctions. Twist is not required for apical Rok recruitment, but instead polarizes Rok medioapically. Therefore, Twist establishes radial cell polarity of Rok/Myo-II and E-cadherin and promotes medioapical actin assembly in mesoderm cells to stabilize cell shape fluctuations.

MeSH Terms
Actins/metabolism Animals Cell Polarity/physiology Cell Shape Drosophila/cytology,embryology Drosophila Proteins/metabolism Inositol/analogs & derivatives,metabolism Intercellular Junctions Myosins/metabolism Signal Transduction Twist-Related Protein 1/metabolism rho GTP-Binding Proteins/metabolism rho-Associated Kinases/metabolism
Chemicals
Actins Drosophila Proteins Twi protein, Drosophila Twist-Related Protein 1 inosose Inositol rho-Associated Kinases Myosins Rho1 protein, Drosophila rho GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mason Frank M
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
Tworoger Michael
Martin Adam C
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1476-4679
DOI
10.1038/ncb2796
Published
2013-08-00
Epub
2013-00-07
Pages
926-36
Language
English
Region
England
NLM ID
100890575
PMCID
PMC3736338
Subset
IM
Grants
NIGMS NIH HHS · K99 GM089826 · United States
NIGMS NIH HHS · R00 GM089826 · United States
NIGMS NIH HHS · R01 GM105984 · United States
NIGMS NIH HHS · R00GM089826 · United States
Corrections
CommentIn
PMID: 24070446
External Links
PubMed source DOI: 10.1038/ncb2796 PMC Full Text View journal details
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