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PMID: 2387629 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of Mycobacterium avium-Mycobacterium intracellulare to human leukocytes.

Infection and immunity ·Vol. 58 ·No. 9 ·1990-09-00 ·Pages 2951-6

Catanzaro A, Wright SD

Abstract

We examined nonopsonic binding of Mycobacterium avium-Mycobacterium intracellulare (MAI) by human leukocytes. Macrophages (M phi) avidly bound fluorescently labeled MAI in the absence of serum proteins. Binding appeared to be mediated by a lineage-specific, proteinaceous receptor on M phi, since (i) binding of labeled bacteria could be competitively inhibited by unlabeled MAI, (ii) treatment of M phi with trypsin ablated the ability of M phi to bind MAI, and (iii) the capacity to bind MAI was observed on monocytes, M phi, and stimulated polymorphonuclear cells but not on lymphocytes or unstimulated polymorphonuclear cells. The receptor for MAI appeared mobile in the plane of the membrane, since spreading of M phi on a carpet of immobilized, unlabeled MAI down modulated binding of labeled MAI added in suspension. The receptor required neither calcium nor magnesium for activity and appeared different from other known receptors for intracellular pathogens.

MeSH Terms
Binding Sites Cell Membrane/drug effects,metabolism Cells, Cultured Humans Kinetics Leukocytes, Mononuclear/drug effects,metabolism Macrophages/drug effects,metabolism Mycobacterium avium Complex/metabolism Neutrophils/drug effects,metabolism T-Lymphocytes/drug effects,metabolism Trypsin/pharmacology
Chemicals
Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Catanzaro A
Department of Medicine, University of California, San Diego 92103.
Wright S D
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24 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.9.2951-2956.1990
Published
1990-09-00
Pages
2951-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313593
Subset
IM
Grants
NIAID NIH HHS · AI 22003 · United States
NIAID NIH HHS · AI 24775 · United States
External Links
PubMed source DOI: 10.1128/iai.58.9.2951-2956.1990 PMC Full Text View journal details
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