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PMID: 238802 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequence organization in the genomes of five marine invertebrates.

Chromosoma ·Vol. 51 ·No. 3 ·1975-07-21 ·Pages 225-51

Goldberg RB, Crain WR, Ruderman JV, Moore GP, Barnett TR, Higgins RC, Gelfand RA, Galau GA, Britten RJ, Davidson EH

Abstract

The arrangement of repetitive and non-repetitive sequence was studied in the genomic DNA of the oyster (Crassostrea virginica), the surf clam (Spisula solidissima), the horseshoe crab (Limulus polyphemus), a nemertean worm (Cerebratulus lacteus) and a jelly-fish (Aurelia aurita). Except for the jellyfish these animals belong to the protostomial branch of animal evolution, for which little information regarding DNA sequence organization has previously been available. The reassociation kinetics of short (250-300 nucleotide) and long (2,000-3,000 nucleotide) DNA fragments was studied by the hydroxyapatite method. It was shown that in each case a major fraction of the DNA consists of single copy sequences less than about 3,000 nucleotides in length, interspersed with short repetitive sequences. The lengths of the repetitive sequences were estimated by optical hyperchromicity and S1 nuclease measurements made on renaturation products. All the genomes studied include a prominent fraction of interspersed repetitive sequences about 300 nucleotides in length, as well as longer repetitive sequence regions.

MeSH Terms
Animals Base Sequence Biological Evolution Bivalvia Brachyura Centrifugation, Density Gradient Chromatography Cnidaria DNA/analysis DNA, Single-Stranded/analysis Deoxyribonucleases Electrophoresis Hydroxyapatites Invertebrates Nucleic Acid Denaturation Nucleic Acid Renaturation Nucleotides/analysis Ostreidae Platyhelminths
Chemicals
DNA, Single-Stranded Hydroxyapatites Nucleotides DNA Deoxyribonucleases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Goldberg R B
Crain W R
Ruderman J V
Moore G P
Barnett T R
Higgins R C
Gelfand R A
Galau G A
Britten R J
Davidson E H
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00284817
Published
1975-07-21
Pages
225-51
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00284817 View journal details
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