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PMID: 23897237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Single-cell sequencing-based technologies will revolutionize whole-organism science.

Nature reviews. Genetics ·Vol. 14 ·No. 9 ·2013-09-00 ·Pages 618-30

Shapiro E, Biezuner T, Linnarsson S

Abstract

The unabated progress in next-generation sequencing technologies is fostering a wave of new genomics, epigenomics, transcriptomics and proteomics technologies. These sequencing-based technologies are increasingly being targeted to individual cells, which will allow many new and longstanding questions to be addressed. For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of marker-based cell types; and single-cell epigenomics and proteomics will allow the functional states of individual cells to be analysed. These technologies will become integrated within a decade or so, enabling high-throughput, multi-dimensional analyses of individual cells that will produce detailed knowledge of the cell lineage trees of higher organisms, including humans. Such studies will have important implications for both basic biological research and medicine.

MeSH Terms
Animals Cell Separation/methods Epigenomics Gene Expression Profiling Genomics High-Throughput Nucleotide Sequencing/methods Humans Proteomics Single-Cell Analysis/methods
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shapiro Ehud
1] Department of Computer Science and Applied Math, Weizmann Institute of Science, Rehovot 76100, Israel. [2] Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
Biezuner Tamir
Linnarsson Sten
References (140)
140 references, click to expand
  1. Tumor self-seeding by circulating cancer cells.
    Cell. 2009 Dec 24;139(7):1315-26 PMID: 20064377
  2. Critical determinants of metastasis.
    Semin Cancer Biol. 2002 Apr;12(2):89-96 PMID: 12027580
  3. An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis.
    Nucleic Acids Res. 2006 Mar 17;34(5):e42 PMID: 16547197
  4. The life history of 21 breast cancers.
    Cell. 2012 May 25;149(5):994-1007 PMID: 22608083
  5. Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling.
    Nucleic Acids Res. 2011 Aug;39(15):6475-88 PMID: 21576221
  6. Post-embryonic cell lineages of the nematode, Caenorhabditis elegans.
    Dev Biol. 1977 Mar;56(1):110-56 PMID: 838129
  7. Extensive somatic microsatellite mutations in normal human tissue.
    Cancer Res. 2001 Jun 1;61(11):4541-4 PMID: 11389087
  8. Methylation reveals a niche: stem cell succession in human colon crypts.
    Oncogene. 2002 Aug 12;21(35):5441-9 PMID: 12154406
  9. Robust 4C-seq data analysis to screen for regulatory DNA interactions.
    Nat Methods. 2012 Oct;9(10):969-72 PMID: 22961246
  10. Single-cell dissection of transcriptional heterogeneity in human colon tumors.
    Nat Biotechnol. 2011 Nov 13;29(12):1120-7 PMID: 22081019
  11. Counting absolute numbers of molecules using unique molecular identifiers.
    Nat Methods. 2011 Nov 20;9(1):72-4 PMID: 22101854
  12. Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes.
    Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1347-52 PMID: 22232676
  13. Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues.
    BMC Biotechnol. 2008 Feb 20;8:17 PMID: 18284708
  14. Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells.
    Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):419-24 PMID: 22203961
  15. Multiplexed protein detection by proximity ligation for cancer biomarker validation.
    Nat Methods. 2007 Apr;4(4):327-9 PMID: 17369836
  16. Comprehensive mapping of long-range interactions reveals folding principles of the human genome.
    Science. 2009 Oct 9;326(5950):289-93 PMID: 19815776
  17. Droplet microfluidics for high-throughput biological assays.
    Lab Chip. 2012 Jun 21;12(12):2146-55 PMID: 22318506
  18. Perspectives and challenges of emerging single-molecule DNA sequencing technologies.
    Small. 2009 Dec;5(23):2638-49 PMID: 19904762
  19. Genome-wide detection of single-nucleotide and copy-number variations of a single human cell.
    Science. 2012 Dec 21;338(6114):1622-6 PMID: 23258894
  20. Direct, genome-wide assessment of DNA mutations in single cells.
    Nucleic Acids Res. 2012 Mar;40(5):2032-40 PMID: 22086961
  21. Cell lineage analysis of the mammalian female germline.
    PLoS Genet. 2012;8(2):e1002477 PMID: 22383887
  22. An integrated map of genetic variation from 1,092 human genomes.
    Nature. 2012 Nov 1;491(7422):56-65 PMID: 23128226
  23. CEL-Seq: single-cell RNA-Seq by multiplexed linear amplification.
    Cell Rep. 2012 Sep 27;2(3):666-73 PMID: 22939981
  24. Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq.
    Genome Res. 2011 Jul;21(7):1160-7 PMID: 21543516
  25. Somatic mutation profiles of MSI and MSS colorectal cancer identified by whole exome next generation sequencing and bioinformatics analysis.
    PLoS One. 2010 Dec 22;5(12):e15661 PMID: 21203531
  26. Target for cancer therapy: proliferating cells or stem cells.
    Leukemia. 2006 Mar;20(3):385-91 PMID: 16357832
  27. Evolution of the cancer genome.
    Nat Rev Genet. 2012 Nov;13(11):795-806 PMID: 23044827
  28. Whole-genome sequencing to delineate Mycobacterium tuberculosis outbreaks: a retrospective observational study.
    Lancet Infect Dis. 2013 Feb;13(2):137-46 PMID: 23158499
  29. A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection.
    Lab Chip. 2011 Apr 7;11(7):1256-61 PMID: 21347466
  30. Targeted capture and massively parallel sequencing of 12 human exomes.
    Nature. 2009 Sep 10;461(7261):272-6 PMID: 19684571
  31. Inferring clonal expansion and cancer stem cell dynamics from DNA methylation patterns in colorectal cancers.
    Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4828-33 PMID: 19261858
  32. Direct RNA sequencing.
    Nature. 2009 Oct 8;461(7265):814-8 PMID: 19776739
  33. Comprehensive mutation analysis for congenital muscular dystrophy: a clinical PCR-based enrichment and next-generation sequencing panel.
    PLoS One. 2013;8(1):e53083 PMID: 23326386
  34. Investigating stem cells in human colon by using methylation patterns.
    Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10839-44 PMID: 11517339
  35. Repeatedly identified differentially expressed proteins (RIDEPs) from antibody microarray proteomic analysis.
    J Proteomics. 2011 May 1;74(5):698-703 PMID: 21338725
  36. Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis.
    Immunity. 2013 Jan 24;38(1):79-91 PMID: 23273845
  37. V(D)J recombination: mechanisms of initiation.
    Annu Rev Genet. 2011;45:167-202 PMID: 21854230
  38. Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells.
    Nature. 2012 Jul 11;487(7406):190-5 PMID: 22785314
  39. A Melanoma Brain Metastasis with a Donor-Patient Hybrid Genome following Bone Marrow Transplantation: First Evidence for Fusion in Human Cancer.
    PLoS One. 2013 Jun 26;8(6):e66731 PMID: 23840523
  40. Analysis of gene expression in single live neurons.
    Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):3010-4 PMID: 1557406
  41. Counting individual DNA molecules by the stochastic attachment of diverse labels.
    Proc Natl Acad Sci U S A. 2011 May 31;108(22):9026-31 PMID: 21562209
  42. Mutational processes molding the genomes of 21 breast cancers.
    Cell. 2012 May 25;149(5):979-93 PMID: 22608084
  43. Central dogma at the single-molecule level in living cells.
    Nature. 2011 Jul 20;475(7356):308-15 PMID: 21776076
  44. In vivo control of CpG and non-CpG DNA methylation by DNA methyltransferases.
    PLoS Genet. 2012 Jun;8(6):e1002750 PMID: 22761581
  45. ProteinSeq: high-performance proteomic analyses by proximity ligation and next generation sequencing.
    PLoS One. 2011;6(9):e25583 PMID: 21980495
  46. Real-time single-molecule co-immunoprecipitation analyses reveal cancer-specific Ras signalling dynamics.
    Nat Commun. 2013;4:1505 PMID: 23422673
  47. Detection and quantification of rare mutations with massively parallel sequencing.
    Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9530-5 PMID: 21586637
  48. Fusion of tumour cells with bone marrow-derived cells: a unifying explanation for metastasis.
    Nat Rev Cancer. 2008 May;8(5):377-86 PMID: 18385683
  49. Cell lineage analysis of acute leukemia relapse uncovers the role of replication-rate heterogeneity and microsatellite instability.
    Blood. 2012 Jul 19;120(3):603-12 PMID: 22645183
  50. Genetic variegation of clonal architecture and propagating cells in leukaemia.
    Nature. 2011 Jan 20;469(7330):356-61 PMID: 21160474
  51. Mitochondrial variability as a source of extrinsic cellular noise.
    PLoS Comput Biol. 2012;8(3):e1002416 PMID: 22412363
  52. Clonal evolution of preleukemic hematopoietic stem cells precedes human acute myeloid leukemia.
    Sci Transl Med. 2012 Aug 29;4(149):149ra118 PMID: 22932223
  53. Intratumor heterogeneity and branched evolution revealed by multiregion sequencing.
    N Engl J Med. 2012 Mar 8;366(10):883-892 PMID: 22397650
  54. Genome-wide identification of in vivo protein-DNA binding sites from ChIP-Seq data.
    Nucleic Acids Res. 2008 Sep;36(16):5221-31 PMID: 18684996
  55. Whole genome amplification from single cells in preimplantation genetic diagnosis and prenatal diagnosis.
    Eur J Obstet Gynecol Reprod Biol. 2007 Mar;131(1):13-20 PMID: 17157976
  56. The embryonic cell lineage of the nematode Caenorhabditis elegans.
    Dev Biol. 1983 Nov;100(1):64-119 PMID: 6684600
  57. Epigenetic polymorphism and the stochastic formation of differentially methylated regions in normal and cancerous tissues.
    Nat Genet. 2012 Nov;44(11):1207-14 PMID: 23064413
  58. Single oocyte bisulfite mutagenesis.
    J Vis Exp. 2012 Jun 27;(64): PMID: 22782232
  59. Use of somatic mutations to quantify random contributions to mouse development.
    BMC Genomics. 2013 Jan 18;14:39 PMID: 23327737
  60. Alternative isoform regulation in human tissue transcriptomes.
    Nature. 2008 Nov 27;456(7221):470-6 PMID: 18978772
  61. Targeted resequencing of candidate genes using selector probes.
    Nucleic Acids Res. 2011 Jan;39(2):e8 PMID: 21059679
  62. The therapeutic promise of the cancer stem cell concept.
    J Clin Invest. 2010 Jan;120(1):41-50 PMID: 20051635
  63. A method for counting PCR template molecules with application to next-generation sequencing.
    Nucleic Acids Res. 2011 Jul;39(12):e81 PMID: 21490082
  64. Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling.
    Nat Protoc. 2011 Apr;6(4):468-81 PMID: 21412275
  65. Detecting antigens by quantitative immuno-PCR.
    Nat Protoc. 2007;2(8):1918-30 PMID: 17703203
  66. Human cell type diversity, evolution, development, and classification with special reference to cells derived from the neural crest.
    Biol Rev Camb Philos Soc. 2006 Aug;81(3):425-55 PMID: 16790079
  67. The future is now: single-cell genomics of bacteria and archaea.
    FEMS Microbiol Rev. 2013 May;37(3):407-27 PMID: 23298390
  68. Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays.
    Lab Chip. 2012 Dec 7;12(23):5007-15 PMID: 23072772
  69. Stem cell transcriptome profiling via massive-scale mRNA sequencing.
    Nat Methods. 2008 Jul;5(7):613-9 PMID: 18516046
  70. Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells.
    Nat Biotechnol. 2012 Aug;30(8):777-82 PMID: 22820318
  71. Ultradeep bisulfite sequencing analysis of DNA methylation patterns in multiple gene promoters by 454 sequencing.
    Cancer Res. 2007 Sep 15;67(18):8511-8 PMID: 17875690
  72. Adult neurogenesis in the mammalian brain: significant answers and significant questions.
    Neuron. 2011 May 26;70(4):687-702 PMID: 21609825
  73. Exome sequencing: the sweet spot before whole genomes.
    Hum Mol Genet. 2010 Oct 15;19(R2):R145-51 PMID: 20705737
  74. Molecular cloning, by a novel approach, of a cDNA encoding a putative olfactory protein in the labial palps of the moth Cactoblastis cactorum.
    Gene. 1997 Nov 20;202(1-2):39-43 PMID: 9427543
  75. Circulating tumor cells: a novel prognostic factor for newly diagnosed metastatic breast cancer.
    J Clin Oncol. 2005 Mar 1;23(7):1420-30 PMID: 15735118
  76. The stem cell population of the human colon crypt: analysis via methylation patterns.
    PLoS Comput Biol. 2007 Mar 2;3(3):e28 PMID: 17335343
  77. Whole-Genome Amplification by Improved Primer Extension Preamplification PCR (I-PEP-PCR).
    CSH Protoc. 2008 Jan 01;2008:pdb.prot4921 PMID: 21356675
  78. Multiplex padlock targeted sequencing reveals human hypermutable CpG variations.
    Genome Res. 2009 Sep;19(9):1606-15 PMID: 19525355
  79. Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm.
    Cell. 2012 Mar 2;148(5):873-85 PMID: 22385957
  80. The transcriptional landscape of the yeast genome defined by RNA sequencing.
    Science. 2008 Jun 6;320(5881):1344-9 PMID: 18451266
  81. Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells.
    Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4494-9 PMID: 10200290
  82. Microfluidic amplification as a tool for massive parallel sequencing of the familial hypercholesterolemia genes.
    Clin Chem. 2012 Apr;58(4):717-24 PMID: 22294733
  83. Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm.
    Cell. 2012 Jul 20;150(2):402-12 PMID: 22817899
  84. Colorectal adenoma and cancer divergence. Evidence of multilineage progression.
    Am J Pathol. 1999 Jun;154(6):1815-24 PMID: 10362806
  85. Identity crisis for adult periventricular neural stem cells: subventricular zone astrocytes, ependymal cells or both?
    Nat Rev Neurosci. 2009 Feb;10(2):153-63 PMID: 19153578
  86. Genome-wide copy number analysis of single cells.
    Nat Protoc. 2012 May 03;7(6):1024-41 PMID: 22555242
  87. Lineage tracing reveals Lgr5+ stem cell activity in mouse intestinal adenomas.
    Science. 2012 Aug 10;337(6095):730-5 PMID: 22855427
  88. Automated analysis of embryonic gene expression with cellular resolution in C. elegans.
    Nat Methods. 2008 Aug;5(8):703-9 PMID: 18587405
  89. Metastasis results from preexisting variant cells within a malignant tumor.
    Science. 1977 Aug 26;197(4306):893-5 PMID: 887927
  90. Phylogenetic fate mapping.
    Proc Natl Acad Sci U S A. 2006 Apr 4;103(14):5448-53 PMID: 16569691
  91. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum.
    Science. 2011 May 6;332(6030):687-96 PMID: 21551058
  92. Maintenance of β-cell maturity and plasticity in the adult pancreas: developmental biology concepts in adult physiology.
    Diabetes. 2012 Jun;61(6):1365-71 PMID: 22618775
  93. Genetic reconstruction of individual colorectal tumor histories.
    Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1236-41 PMID: 10655514
  94. Single-molecule DNA sequencing of a viral genome.
    Science. 2008 Apr 4;320(5872):106-9 PMID: 18388294
  95. Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing.
    Science. 2012 Dec 21;338(6114):1627-30 PMID: 23258895
  96. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14676-9 PMID: 8962113
  97. Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases.
    Nat Neurosci. 2004 Feb;7(2):136-44 PMID: 14703572
  98. Development and optimization of a process for automated recovery of single cells identified by microengraving.
    Biotechnol Prog. 2010 May-Jun;26(3):888-95 PMID: 20063389
  99. Mapping and quantifying mammalian transcriptomes by RNA-Seq.
    Nat Methods. 2008 Jul;5(7):621-8 PMID: 18516045
  100. Nature, nurture, or chance: stochastic gene expression and its consequences.
    Cell. 2008 Oct 17;135(2):216-26 PMID: 18957198
  101. Tumour evolution inferred by single-cell sequencing.
    Nature. 2011 Apr 7;472(7341):90-4 PMID: 21399628
  102. The evolution of cell types in animals: emerging principles from molecular studies.
    Nat Rev Genet. 2008 Nov;9(11):868-82 PMID: 18927580
  103. Multidimensional regulation of gene expression in the C. elegans embryo.
    Genome Res. 2012 Jul;22(7):1282-94 PMID: 22508763
  104. Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor.
    Cell. 2012 Mar 2;148(5):886-95 PMID: 22385958
  105. Characterizing and measuring bias in sequence data.
    Genome Biol. 2013 May 29;14(5):R51 PMID: 23718773
  106. Microfluidic single cell analysis: from promise to practice.
    Curr Opin Chem Biol. 2012 Aug;16(3-4):381-90 PMID: 22525493
  107. Targeted genomic sequencing of pediatric Burkitt lymphoma identifies recurrent alterations in antiapoptotic and chromatin-remodeling genes.
    Blood. 2012 Dec 20;120(26):5181-4 PMID: 23091298
  108. Whole-exome sequencing efficiently detects rare mutations in autosomal recessive nonsyndromic hearing loss.
    PLoS One. 2012;7(11):e50628 PMID: 23226338
  109. Decoding cell lineage from acquired mutations using arbitrary deep sequencing.
    Nat Methods. 2011 Nov 27;9(1):78-80 PMID: 22120468
  110. Microdroplet-based PCR enrichment for large-scale targeted sequencing.
    Nat Biotechnol. 2009 Nov;27(11):1025-31 PMID: 19881494
  111. Comprehensive human genome amplification using multiple displacement amplification.
    Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5261-6 PMID: 11959976
  112. A window into third-generation sequencing.
    Hum Mol Genet. 2010 Oct 15;19(R2):R227-40 PMID: 20858600
  113. Initial sequencing and analysis of the human genome.
    Nature. 2001 Feb 15;409(6822):860-921 PMID: 11237011
  114. High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire.
    Nat Biotechnol. 2013 Feb;31(2):166-9 PMID: 23334449
  115. Muscle-bound primordial stem cells give rise to myofiber-associated myogenic and non-myogenic progenitors.
    PLoS One. 2011;6(10):e25605 PMID: 22022423
  116. Visualization of omics data for systems biology.
    Nat Methods. 2010 Mar;7(3 Suppl):S56-68 PMID: 20195258
  117. Optical tweezers for single cells.
    J R Soc Interface. 2008 Jul 6;5(24):671-90 PMID: 18381254
  118. Microsatellites: simple sequences with complex evolution.
    Nat Rev Genet. 2004 Jun;5(6):435-45 PMID: 15153996
  119. Genomic analysis at the single-cell level.
    Annu Rev Genet. 2011;45:431-45 PMID: 21942365
  120. Phylogenetic analysis of developmental and postnatal mouse cell lineages.
    Evol Dev. 2010 Jan-Feb;12(1):84-94 PMID: 20156285
  121. Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis.
    Nat Protoc. 2007;2(3):739-52 PMID: 17406636
  122. Laser capture microdissection of fluorescently labeled embryonic cranial neural crest cells.
    Genesis. 2004 May;39(1):58-64 PMID: 15124228
  123. Capturing chromosome conformation.
    Science. 2002 Feb 15;295(5558):1306-11 PMID: 11847345
  124. Whole-genome molecular haplotyping of single cells.
    Nat Biotechnol. 2011 Jan;29(1):51-7 PMID: 21170043
  125. A high-throughput DNA methylation analysis of a single cell.
    Nucleic Acids Res. 2011 Apr;39(7):e44 PMID: 21266484
  126. Reconstruction of cell lineage trees in mice.
    PLoS One. 2008 Apr 09;3(4):e1939 PMID: 18398465
  127. Colon stem cell and crypt dynamics exposed by cell lineage reconstruction.
    PLoS Genet. 2011 Jul;7(7):e1002192 PMID: 21829376
  128. Estimating cell depth from somatic mutations.
    PLoS Comput Biol. 2008 May 09;4(4):e1000058 PMID: 18404205
  129. Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity.
    Genome Biol. 2013 Apr 17;14(4):R31 PMID: 23594475
  130. Distant metastasis occurs late during the genetic evolution of pancreatic cancer.
    Nature. 2010 Oct 28;467(7319):1114-7 PMID: 20981102
  131. Library-free methylation sequencing with bisulfite padlock probes.
    Nat Methods. 2012 Feb 05;9(3):270-2 PMID: 22306810
  132. Molecular live cell bioimaging in stem cell research.
    Ann N Y Acad Sci. 2012 Aug;1266:18-27 PMID: 22901252
  133. High-throughput microfluidic single-cell RT-qPCR.
    Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):13999-4004 PMID: 21808033
  134. Toward clinical proteomics on a next-generation sequencing platform.
    Anal Chem. 2011 Feb 1;83(3):666-70 PMID: 21142014
  135. Quantification of cardiovascular biomarkers in patient plasma by targeted mass spectrometry and stable isotope dilution.
    Mol Cell Proteomics. 2009 Oct;8(10):2339-49 PMID: 19596694
  136. Cancer stem cells: Here, there, everywhere?
    Nature. 2008 Dec 4;456(7222):581-2 PMID: 19052611
  137. Clonal evolution in relapsed acute myeloid leukaemia revealed by whole-genome sequencing.
    Nature. 2012 Jan 11;481(7382):506-10 PMID: 22237025
  138. Probing cellular protein complexes using single-molecule pull-down.
    Nature. 2011 May 26;473(7348):484-8 PMID: 21614075
  139. mRNA-Seq whole-transcriptome analysis of a single cell.
    Nat Methods. 2009 May;6(5):377-82 PMID: 19349980
  140. Real-time DNA sequencing from single polymerase molecules.
    Science. 2009 Jan 2;323(5910):133-8 PMID: 19023044
Article Info
Journal
Nature reviews. Genetics
Abbr.
Nat Rev Genet
ISSN
1471-0064
DOI
10.1038/nrg3542
Published
2013-09-00
Epub
2013-00-30
Pages
618-30
Language
English
Region
England
NLM ID
100962779
Subset
IM
External Links
PubMed source DOI: 10.1038/nrg3542 View journal details
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