A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley.
We describe the nucleotide sequence and some structural characteristics of a single copy gene encoding pathogenesis-related protein 2 (PR2) in parsley (Petroselinum crispum). Transcriptional activation of this gene in cultured parsley cells treated with fungal elicitor leads to a rapid, large and transient accumulation of PR2 mRNA. The deduced PR2 protein belongs to a novel class of evolutionarily conserved polypeptides which are closely related to disease resistance in plants. Functional analysis of a series of truncated PR2 promoter fusions with the beta-glucuronidase reporter gene, using parsley protoplasts for transient expression studies, identified a 5' upstream element between positions -168 and -52 necessary for strong elicitor responsiveness. This small promoter fragment is active in conjunction with its own TATA box region as well as with the corresponding region from a heterologous promoter. The PR2 regulatory region exhibits no sequence similarity to any other elicitor-responsive promoter known to date.
21 references, click to expand
-
Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor.EMBO J. 1987 Sep;6(9):2551-6 PMID: 16453792
-
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.DNA. 1985 Apr;4(2):165-70 PMID: 3996185
-
Primary structures and catalytic properties of isoenzymes encoded by the two 4-coumarate: CoA ligase genes in parsley.Eur J Biochem. 1988 Oct 1;176(3):661-7 PMID: 3169018
-
Induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in cultured plant cells by UV light or fungal elicitor.Proc Natl Acad Sci U S A. 1984 Feb;81(4):1102-6 PMID: 16593418
-
Coordinated regulation of 4-coumarate:CoA ligase and phenylalanine ammonia-lyase mRNAs in cultured plant cells.J Biol Chem. 1981 Oct 10;256(19):10061-5 PMID: 7275967
-
Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco.Plant Cell. 1989 Jun;1(6):599-607 PMID: 2535512
-
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
-
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.Proc Natl Acad Sci U S A. 1986 Nov;83(22):8447-51 PMID: 3534890
-
Host-Pathogen Interactions: IX. Quantitative Assays of Elicitor Activity and Characterization of the Elicitor Present in the Extracellular Medium of Cultures of Phytophthora megasperma var. sojae.Plant Physiol. 1976 May;57(5):751-9 PMID: 16659565
-
A phenylalanine ammonia-lyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements.EMBO J. 1989 Jun;8(6):1641-8 PMID: 2767049
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
-
The isolation of nuclei from tissue-cultured plant cells.Exp Cell Res. 1981 Sep;135(1):69-77 PMID: 6269864
-
Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene.EMBO J. 1989 Mar;8(3):651-6 PMID: 2566481
-
Structure and elicitor or u.v.-light-stimulated expression of two 4-coumarate:CoA ligase genes in parsley.EMBO J. 1987 May;6(5):1189-95 PMID: 16453765
-
Glutathione and fungal elicitor regulation of a plant defense gene promoter in electroporated protoplasts.Proc Natl Acad Sci U S A. 1988 Sep;85(18):6738-42 PMID: 16593981
-
Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley.Mol Gen Genet. 1988 Jul;213(1):93-8 PMID: 3221838
-
The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene.EMBO J. 1989 Jul;8(7):1935-8 PMID: 2571499
-
Signals and transduction mechanisms for activation of plant defenses against microbial attack.Cell. 1989 Jan 27;56(2):215-24 PMID: 2643475
-
Rapid activation by fungal elicitor of genes encoding "pathogenesis-related" proteins in cultured parsley cells.Proc Natl Acad Sci U S A. 1986 Apr;83(8):2427-30 PMID: 16593684
-
Functional architecture of the light-responsive chalcone synthase promoter from parsley.Plant Cell. 1989 Jul;1(7):707-14 PMID: 2535519
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