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PMID: 23913002 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions.

Nature genetics ·Vol. 45 ·No. 9 ·2013-09-00 ·Pages 1097-102

Uga Y, Sugimoto K, Ogawa S, Rane J, Ishitani M, Hara N, Kitomi Y, Inukai Y, Ono K, Kanno N, Inoue H, Takehisa H, Motoyama R, Nagamura Y, Wu J, Matsumoto T, Takai T, Okuno K, Yano M

Abstract

The genetic improvement of drought resistance is essential for stable and adequate crop production in drought-prone areas. Here we demonstrate that alteration of root system architecture improves drought avoidance through the cloning and characterization of DEEPER ROOTING 1 (DRO1), a rice quantitative trait locus controlling root growth angle. DRO1 is negatively regulated by auxin and is involved in cell elongation in the root tip that causes asymmetric root growth and downward bending of the root in response to gravity. Higher expression of DRO1 increases the root growth angle, whereby roots grow in a more downward direction. Introducing DRO1 into a shallow-rooting rice cultivar by backcrossing enabled the resulting line to avoid drought by increasing deep rooting, which maintained high yield performance under drought conditions relative to the recipient cultivar. Our experiments suggest that control of root system architecture will contribute to drought avoidance in crops.

MeSH Terms
Adaptation, Biological/genetics Droughts Gene Expression Regulation, Plant/drug effects Gene Order Genes, Plant Genotype Molecular Sequence Data Oryza/genetics,growth & development Phenotype Plant Growth Regulators/pharmacology Plant Roots/cytology,genetics,growth & development Quantitative Trait Loci
Chemicals
Plant Growth Regulators
Authors & Affiliations
19 authors, click to expand affiliations / ORCID
Uga Yusaku
National Institute of Agrobiological Sciences, Tsukuba, Japan. [email protected]
Sugimoto Kazuhiko
Ogawa Satoshi
Rane Jagadish
Ishitani Manabu
Hara Naho
Kitomi Yuka
Inukai Yoshiaki
Ono Kazuko
Kanno Noriko
Inoue Haruhiko
Takehisa Hinako
Motoyama Ritsuko
Nagamura Yoshiaki
Wu Jianzhong
Matsumoto Takashi
Takai Toshiyuki
Okuno Kazutoshi
Yano Masahiro
References (33)
33 references, click to expand
  1. Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling.
    Plant Cell. 2005 May;17(5):1387-96 PMID: 15829602
  2. ARL1, a LOB-domain protein required for adventitious root formation in rice.
    Plant J. 2005 Jul;43(1):47-56 PMID: 15960615
  3. Adventitious root formation in rice requires OsGNOM1 and is mediated by the OsPINs family.
    Cell Res. 2009 Sep;19(9):1110-9 PMID: 19546891
  4. Characterization of OsPID, the rice ortholog of PINOID, and its possible involvement in the control of polar auxin transport.
    Plant Cell Physiol. 2007 Mar;48(3):540-9 PMID: 17303594
  5. Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice.
    Plant Cell Physiol. 2008 Oct;49(10):1429-50 PMID: 18718932
  6. Compensation and interaction between RISBZ1 and RPBF during grain filling in rice.
    Plant J. 2009 Sep;59(6):908-20 PMID: 19473328
  7. Simple RNAi vectors for stable and transient suppression of gene function in rice.
    Plant Cell Physiol. 2004 Apr;45(4):490-5 PMID: 15111724
  8. The Rice Annotation Project Database (RAP-DB): 2008 update.
    Nucleic Acids Res. 2008 Jan;36(Database issue):D1028-33 PMID: 18089549
  9. Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH4(+) concentrations in hydroponic conditions.
    Theor Appl Genet. 2010 Aug;121(3):535-47 PMID: 20390245
  10. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements.
    Plant Cell. 1997 Nov;9(11):1963-71 PMID: 9401121
  11. Analysis of changes in relative elemental growth rate patterns in the elongation zone of Arabidopsis roots upon gravistimulation.
    Planta. 1998 Nov;206(4):598-603 PMID: 9821690
  12. Gravity sensing and signaling.
    Curr Opin Plant Biol. 2004 Dec;7(6):712-8 PMID: 15491921
  13. Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal.
    Nat Cell Biol. 2005 Nov;7(11):1057-65 PMID: 16244669
  14. The auxin responsive AP2/ERF transcription factor CROWN ROOTLESS5 is involved in crown root initiation in rice through the induction of OsRR1, a type-A response regulator of cytokinin signaling.
    Plant J. 2011 Aug;67(3):472-84 PMID: 21481033
  15. Six-rowed barley originated from a mutation in a homeodomain-leucine zipper I-class homeobox gene.
    Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1424-9 PMID: 17220272
  16. Dro1, a major QTL involved in deep rooting of rice under upland field conditions.
    J Exp Bot. 2011 May;62(8):2485-94 PMID: 21212298
  17. Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed.
    Nat Protoc. 2008;3(5):824-34 PMID: 18451790
  18. Field transcriptome revealed critical developmental and physiological transitions involved in the expression of growth potential in japonica rice.
    BMC Plant Biol. 2011 Jan 12;11:10 PMID: 21226959
  19. Comprehensive expression profiling analysis of OsIAA gene family in developmental processes and in response to phytohormone and stress treatments.
    Planta. 2009 Feb;229(3):577-91 PMID: 19034497
  20. Ehd3, encoding a plant homeodomain finger-containing protein, is a critical promoter of rice flowering.
    Plant J. 2011 May;66(4):603-12 PMID: 21284756
  21. Nuclear ubiquitin proteasome degradation affects WRKY45 function in the rice defense program.
    Plant J. 2013 Jan;73(2):302-13 PMID: 23013464
  22. Evaluation of near-isogenic lines for drought resistance QTL and fine mapping of a locus affecting flag leaf width, spikelet number, and root volume in rice.
    Theor Appl Genet. 2011 Sep;123(5):815-26 PMID: 21681490
  23. Genetic control of root development in rice, the model cereal.
    Trends Plant Sci. 2010 Apr;15(4):219-26 PMID: 20153971
  24. Auxin: a trigger for change in plant development.
    Cell. 2009 Mar 20;136(6):1005-16 PMID: 19303845
  25. Genome-wide transcriptome dissection of the rice root system: implications for developmental and physiological functions.
    Plant J. 2012 Jan;69(1):126-40 PMID: 21895812
  26. OsARF1, an auxin response factor from rice, is auxin-regulated and classifies as a primary auxin responsive gene.
    Plant Mol Biol. 2002 Oct;50(3):415-25 PMID: 12369618
  27. Identification of qSOR1, a major rice QTL involved in soil-surface rooting in paddy fields.
    Theor Appl Genet. 2012 Jan;124(1):75-86 PMID: 21894467
  28. Plant genetics. The blue revolution, drop by drop, gene by gene.
    Science. 2008 Apr 11;320(5873):171-3 PMID: 18403686
  29. Auxin-responsive gene expression: genes, promoters and regulatory factors.
    Plant Mol Biol. 2002 Jun-Jul;49(3-4):373-85 PMID: 12036261
  30. Genome-wide analysis of the auxin response factors (ARF) gene family in rice (Oryza sativa).
    Gene. 2007 Jun 1;394(1-2):13-24 PMID: 17408882
  31. The ethylene response factors SNORKEL1 and SNORKEL2 allow rice to adapt to deep water.
    Nature. 2009 Aug 20;460(7258):1026-30 PMID: 19693083
  32. A novel sensor to map auxin response and distribution at high spatio-temporal resolution.
    Nature. 2012 Jan 15;482(7383):103-6 PMID: 22246322
  33. Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions.
    Plant Cell Physiol. 2002 Oct;43(10):1096-105 PMID: 12407188
Article Info
Journal
Nature genetics
Abbr.
Nat Genet
ISSN
1546-1718
DOI
10.1038/ng.2725
Email
[email protected]
Published
2013-09-00
Epub
2013-00-04
Pages
1097-102
Language
English
Region
United States
NLM ID
9216904
Subset
IM
Databases
GENBANK
AB689741, AB689742
GEO
GSE46255
External Links
PubMed source DOI: 10.1038/ng.2725 View journal details
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