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PMID: 23948253 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Exo70 generates membrane curvature for morphogenesis and cell migration.

Developmental cell ·Vol. 26 ·No. 3 ·2013-08-12 ·Pages 266-78

Zhao Y, Liu J, Yang C, Capraro BR, Baumgart T, Bradley RP, Ramakrishnan N, Xu X, Radhakrishnan R, Svitkina T, Guo W

Abstract

Dynamic shape changes of the plasma membrane are fundamental to many processes, ranging from morphogenesis and cell migration to phagocytosis and viral propagation. Here, we demonstrate that Exo70, a component of the exocyst complex, induces tubular membrane invaginations toward the lumen of synthetic vesicles in vitro and generates protrusions on the surface of cells. Biochemical analyses using Exo70 mutants and independent molecular dynamics simulations based on Exo70 structure demonstrate that Exo70 generates negative membrane curvature through an oligomerization-based mechanism. In cells, the membrane-deformation function of Exo70 is required for protrusion formation and directional cell migration. Exo70 thus represents a membrane-bending protein that may couple actin dynamics and plasma membrane remodeling for morphogenesis.

MeSH Terms
Actin Cytoskeleton/physiology,ultrastructure Actins/physiology Animals Cell Line, Tumor Cell Membrane/physiology Cell Movement/physiology Computer Simulation Dimerization Green Fluorescent Proteins HEK293 Cells Humans Melanoma Membrane Proteins/physiology Mice Microscopy, Electron Microscopy, Fluorescence Models, Biological Morphogenesis/physiology Pseudopodia/physiology,ultrastructure Time-Lapse Imaging Vesicular Transport Proteins/chemistry,physiology
Chemicals
Actins EXOC7 protein, human Exo70 protein, mouse Membrane Proteins Vesicular Transport Proteins Green Fluorescent Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Zhao Yuting
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
Liu Jianglan
Yang Changsong
Capraro Benjamin R
Baumgart Tobias
Bradley Ryan P
Ramakrishnan N
Xu Xiaowei
Radhakrishnan Ravi
Svitkina Tatyana
Guo Wei
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2013.07.007
Published
2013-08-12
Pages
266-78
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC3762978
Subset
IM
Grants
NCRR NIH HHS · S10 RR022482 · United States
NCRR NIH HHS · RR-22482 · United States
NIGMS NIH HHS · GM085146 · United States
NIGMS NIH HHS · R01 GM085146 · United States
NIGMS NIH HHS · T32 GM008275 · United States
External Links
PubMed source DOI: 10.1016/j.devcel.2013.07.007 PMC Full Text View journal details
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