Home LiteratureArticle Details
PMID: 239708 Published · ppublish English Journal Article

The formation of mono-N-acetylhexosamine derivatives of dolichol diphosphate by pig liver microsomal fractions.

The Biochemical journal ·Vol. 148 ·No. 2 ·1975-05-00 ·Pages 245-51

Palamarczyk G, Hemming FW

Abstract

Incubation of pig liver microsomal preparations with UDP-N[U-14C]acetylglucosamine yields a 14C-labelled lipid. The requirement for Mn2+, the pH optimum, time-dependence and the reversibility by UMP of the transferase are reported. Evidence is presented in favour of the lipid being a mixture of dolichol diphosphate N-[14C]acetylglucosamine and dolichol diphosphate N-[14C]acetylmannosamine. Available data suggest that the epimerization takes place while the hexosamine is bound in this lipid-soluble form. The N-acetylmannosamine appeared not be be released into the medium. The subfractionation of the microsomal fraction to separate transferase activity from membrane-bound beta-N-acetylglucosaminidase activity is also reported.

MeSH Terms
Animals Dolichols/analogs & derivatives,metabolism Hydrogen-Ion Concentration In Vitro Techniques Manganese Microsomes, Liver/metabolism Swine Time Factors Uracil Nucleotides/metabolism Uridine Diphosphate N-Acetylglucosamine/metabolism Uridine Diphosphate Sugars/metabolism
Chemicals
Dolichols Uracil Nucleotides Uridine Diphosphate Sugars Manganese Uridine Diphosphate N-Acetylglucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Palamarczyk G
Hemming F W
References (14)
14 references, click to expand
  1. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. The role of dolichol monophosphate in sugar transfer.
    Arch Biochem Biophys. 1971 Apr;143(2):375-83 PMID: 5558132
  4. The unambiguous characterization of dolichol phosphate mannose as a product of mannosyl transferase in pig liver endoplasmic reticulum.
    FEBS Lett. 1973 May 1;31(3):335-8 PMID: 4354061
  5. Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.
    J Cell Biol. 1973 Oct;59(1):45-72 PMID: 4356571
  6. Biosynthesis of a P1-2-acetamido-2-deoxy-D-glucosyl P2-polyisoprenyl pyrophosphate by calf pancreas microsomes.
    Biochem Biophys Res Commun. 1974 Jan 23;56(2):407-14 PMID: 4363053
  7. Incorporation of carbohydrates into endogenous acceptors of liver microsomal fractions.
    Arch Biochem Biophys. 1970 May;138(1):135-46 PMID: 4315690
  8. Mannose transfer to lipid linked di-N-acetylchitobiose.
    Biochem Biophys Res Commun. 1974 Sep 9;60(1):118-25 PMID: 4423204
  9. The biosynthesis of a N,N'-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative.
    Biochem Biophys Res Commun. 1973 Jun 19;52(4):1285-92 PMID: 4717748
  10. The transfer of mannose from guanosine diphosphate mannose to dolichol phosphate and protein by pig liver endoplasmic reticulum.
    Biochem J. 1972 Nov;130(1):77-93 PMID: 4655455
  11. Polyprenol phosphate as an acceptor of mannose from guanosine diphosphate mannose in Aspergillus niger.
    Biochem J. 1972 Mar;126(5):1203-8 PMID: 5073732
  12. Phosphoryl-N-acetylglucosamine transfer to a lipid acceptor of liver microsomal preparations.
    Biochim Biophys Acta. 1971 Sep 1;239(3):401-10 PMID: 5113503
  13. Isolation of rat liver plasma membranes. Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes.
    J Cell Biol. 1970 Dec;47(3):604-18 PMID: 5497542
  14. Studies onthe mechanism of action of uridine diphosphate N-acetylglucosamine 2-epimerase.
    Biochemistry. 1970 Feb 17;9(4):882-5 PMID: 5417402
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1480245
Published
1975-05-00
Pages
245-51
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165532
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1480245 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2