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PMID: 24009651 Published · epublish English Journal Article

In vitro electrical-stimulated wound-healing chip for studying electric field-assisted wound-healing process.

Biomicrofluidics ·Vol. 6 ·No. 3 ·2012-00-00 ·Pages 34117

Sun YS, Peng SW, Cheng JY

Abstract

The wound-healing assay is an easy and economical way to quantify cell migration under diverse stimuli. Traditional assays such as scratch assays and barrier assays are widely and commonly used, but neither of them can represent the complicated condition when a wound occurs. It has been suggested that wound-healing is related to electric fields, which were found to regulate wound re-epithelialization. As a wound occurs, the disruption of epithelial barrier short-circuits the trans-epithelial potential and then a lateral endogenous electric field is created. This field has been proved invitro as an important cue for guiding the migration of fibroblasts, macrophages, and keratinocytes, a phenomenon termed electrotaxis or galvanotaxis. In this paper, we report a microfluidic electrical-stimulated wound-healing chip (ESWHC) integrating electric field with a modified barrier assay. This chip was used to study the migration of fibroblasts under different conditions such as serum, electric field, and wound-healing-promoting drugs. We successfully demonstrate the feasibility of ESWHC to effectively and quantitatively study cell migration during wound-healing process, and therefore this chip could be useful in drug discovery and drug safety tests.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sun Yung-Shin
Research Center for Applied Sciences, Academia Sinica, Taipei City 11529, Taiwan.
Peng Shih-Wei
Cheng Ji-Yen
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Article Info
Journal
Biomicrofluidics
Abbr.
Biomicrofluidics
ISSN
1932-1058
DOI
10.1063/1.4750486
Published
2012-00-00
Epub
2012-00-05
Pages
34117
Language
English
Region
United States
NLM ID
101293825
PMCID
PMC3448595
External Links
PubMed source DOI: 10.1063/1.4750486 PMC Full Text View journal details
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