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PMID: 2404875 Published · ppublish English Journal Article

Cloning and expression of the gene for the cross-reactive alpha antigen of Mycobacterium kansasii.

Infection and immunity ·Vol. 58 ·No. 2 ·1990-02-00 ·Pages 550-6

Matsuo K, Yamaguchi R, Yamazaki A, Tasaka H, Terasaka K, Yamada T

Abstract

The gene for the extracellular alpha antigen of Mycobacterium kansasii was cloned by using the alpha-antigen gene fragments of M. bovis BCG as probes. Gene analysis revealed that this gene encodes 325 amino acid residues, including 40 amino acids for the signal peptide, followed by 285 amino acids for the mature protein. A comparison of the nucleotide sequences of the genes isolated from these two mycobacterial species showed that the levels of DNA and amino acid homology were 84.8 and 89.1%, respectively. The hydropathy profiles were also compared, and two highly changed hydrophilic regions were observed, which might account for the antigenic diversity of this antigen or its acquirement of antigenic specificity.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/analysis,genetics,immunology Base Sequence Blotting, Southern Cloning, Molecular Cross Reactions Escherichia coli/genetics Fibronectins/metabolism Genes, Bacterial Molecular Sequence Data Mycobacterium/genetics Nontuberculous Mycobacteria/genetics,immunology
Chemicals
Antigens, Bacterial Fibronectins alpha antigens, Mycobacterium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Matsuo K
Central Research Laboratories of Ajinomoto Co. Inc., Kawasaki City, Japan.
Yamaguchi R
Yamazaki A
Tasaka H
Terasaka K
Yamada T
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.2.550-556.1990
Published
1990-02-00
Pages
550-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258492
Subset
IM
Databases
GENBANK
X53897
External Links
PubMed source DOI: 10.1128/iai.58.2.550-556.1990 PMC Full Text View journal details
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