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PMID: 24077441 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid.

Reproductive sciences (Thousand Oaks, Calif.) ·Vol. 21 ·No. 5 ·2014-05-00 ·Pages 573-81

Sati L, Cayli S, Delpiano E, Sakkas D, Huszar G

Abstract

In mammalian species, acquisition of sperm fertilization competence is dependent on the phenomenon of sperm capacitation. One of the key elements of capacitation is protein tyrosine phosphorylation (TP) in various sperm membrane regions. In previous studies performed, the pattern of TP was examined in human sperm bound to zona pellucida of oocytes. In the present comparative study, TP patterns upon sperm binding to the zona pellucida or hyaluronic acid (HA) were investigated in spermatozoa arising from the same semen samples. Tyrosine phosphorylation, visualized by immunofluorescence, was localized within the acrosomal cap, equatorial head region, neck, and the principal piece. Tyrosine phosphorylation has increased in a time-related manner as capacitation progressed, and the phosphorylation pattern was identical within the principal piece and neck, regardless of the sperm bound to the zona pellucida or HA. Thus, the data demonstrated that the patterns of sperm activation-related TP were similar regardless of the spermatozoa bound to zona pellucida or HA. Further, sperm with incomplete development, as detected by excess cytoplasmic retention, failed to exhibit TP.

Keywords
capacitation human sperm hyaluronic acid phosphotyrosine zona pellucida
MeSH Terms
Humans Hyaluronic Acid/metabolism Male Phosphorylation/physiology Protein Binding/physiology Sperm Motility/physiology Spermatozoa/metabolism Tyrosine/metabolism Zona Pellucida/metabolism
Chemicals
Tyrosine Hyaluronic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sati Leyla
1Department of Histology and Embryology, Akdeniz University School of Medicine, Antalya, Turkey.
Cayli Sevil
Delpiano Elena
Sakkas Denny
Huszar Gabor
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Article Info
Journal
Reproductive sciences (Thousand Oaks, Calif.)
Abbr.
Reprod Sci
ISSN
1933-7205
DOI
10.1177/1933719113504467
Published
2014-05-00
Epub
2013-00-27
Pages
573-81
Language
English
Region
United States
NLM ID
101291249
PMCID
PMC3984481
Subset
IM
Grants
NIOSH CDC HHS · R01 OH004061 · United States
NIOSH CDC HHS · OH-04061 · United States
External Links
PubMed source DOI: 10.1177/1933719113504467 PMC Full Text View journal details
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