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PMID: 2408042 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

lac repressor: crystallization of intact tetramer and its complexes with inducer and operator DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 5 ·1990-03-00 ·Pages 1870-3

Pace HC, Lu P, Lewis M

Abstract

The intact lac repressor tetramer, which regulates expression of the lac operon in Escherichia coli, has been crystallized in the native form, with an inducer, and in a ternary complex with operator DNA and an anti-inducer. The crystals without DNA diffract to better than 3.5 A. They belong to the monoclinic space group C2 and have cell dimensions a = 164.7 A, b = 75.6 A, and c = 161.2 A, with alpha = gamma = 90 degrees and beta = 125.5 degrees. Cocrystals have been obtained with a number of different lac operator-related DNA fragments. The complex with a blunt-ended 16-base-pair strand yielded tetragonal bipyramids that diffract to 6.5 A. These protein-DNA cocrystals crack upon exposure to the gratuitous inducer isopropyl beta-D-thiogalactoside, suggesting a conformational change in the repressor-operator complex.

MeSH Terms
Base Sequence Chromatography, High Pressure Liquid Crystallization DNA, Bacterial/isolation & purification,metabolism Escherichia coli/genetics,metabolism Gene Expression Regulation, Bacterial Lac Operon Macromolecular Substances Molecular Sequence Data Oligodeoxyribonucleotides/chemical synthesis,isolation & purification Protein Binding Repressor Proteins/isolation & purification,metabolism Transcription Factors/metabolism X-Ray Diffraction
Chemicals
DNA, Bacterial Macromolecular Substances Oligodeoxyribonucleotides Repressor Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pace H C
Department of Chemistry, University of Pennsylvania, Philadelphia 19104.
Lu P
Lewis M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.5.1870
Published
1990-03-00
Pages
1870-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53585
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.5.1870 PMC Full Text View journal details
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