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PMID: 2409666 Published · ppublish English Comparative Study Journal Article

Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5).

Virchows Archiv. A, Pathological anatomy and histopathology ·Vol. 407 ·No. 1 ·1985-00-00 ·Pages 1-12

von Overbeck J, Stähli C, Gudat F, Carmann H, Lautenschlager C, Dürmüller U, Takacs B, Miggiano V, Staehelin T, Heitz PU

Abstract

A mouse monoclonal antibody (mAB lu-5) was prepared using a lung cancer cell line as an antigen. The selected clone produces an IgG with a gamma-1 heavy chain and a kappa-light-chain. Immunohistochemical testing of mAB lu-5 on 117 normal tissue biopsies and 474 tumours revealed reactivity with an intracytoplasmic, formaldehyderesistant antigen present in most epithelial and mesothelial cells, but absent in mesenchymal cells. The antibody can therefore be used as a first order, pan-epithelial marker. It proved also useful for fast tumour diagnosis on frozen sections.

MeSH Terms
Animals Antibodies, Monoclonal Carcinoma/immunology,pathology Epithelial Cells Epithelium/immunology Epitopes/analysis Female Fluorescent Antibody Technique Freezing Histological Techniques Humans Immunoenzyme Techniques Immunoglobulin G/analysis Male Mice Mice, Inbred BALB C Neoplasms/immunology,pathology Paraffin
Chemicals
Antibodies, Monoclonal Epitopes Immunoglobulin G Paraffin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
von Overbeck J
Stähli C
Gudat F
Carmann H
Lautenschlager C
Dürmüller U
Takacs B
Miggiano V
Staehelin T
Heitz P U
References (20)
20 references, click to expand
  1. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY.
    Virology. 1963 Aug;20:642-4 PMID: 14059832
  2. Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections.
    Lab Invest. 1975 Jul;33(1):88-95 PMID: 1095822
  3. Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Use in surgical pathology.
    Lab Invest. 1983 Sep;49(3):353-61 PMID: 6193333
  4. Immunohistochemistry of soft tissue tumors: progress and prospects.
    Hum Pathol. 1982 Nov;13(11):969-74 PMID: 6295916
  5. Distribution of tissue polypeptide antigen (TPA) in normal human tissues: Immunohistochemical study on unfixed, methanol-, ethanol-, and formalin-fixed tissues.
    J Histochem Cytochem. 1985 Feb;33(2):99-109 PMID: 3968422
  6. Immunoperoxidase localization of prostate-specific antigen.
    Am J Surg Pathol. 1982 Sep;6(6):553-7 PMID: 6183983
  7. Cytokeratins of normal epithelia and some neoplasms of the female genital tract.
    Lab Invest. 1983 Nov;49(5):599-610 PMID: 6195450
  8. Antibodies to different intermediate filaments as histogenetic tumormarkers.
    Acta Histochem Suppl. 1984;29:129-36 PMID: 6201951
  9. Immunoreactivity of paraffin-embedded normal tissues and mesenchymal tumors for smooth muscle myosin.
    Am J Clin Pathol. 1983 Nov;80(5):677-81 PMID: 6637880
  10. The use of avidin-biotin interaction in immunoenzymatic techniques.
    J Histochem Cytochem. 1979 Aug;27(8):1131-9 PMID: 90074
  11. Spleen cell analysis and optimal immunization for high-frequency production of specific hybridomas.
    Methods Enzymol. 1983;92:26-36 PMID: 6304456
  12. Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody.
    EMBO J. 1982;1(11):1429-37 PMID: 6202509
  13. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
    J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
  14. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells.
    Int J Cancer. 1976 Jan 15;17(1):62-70 PMID: 175022
  15. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  16. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
    Cell. 1982 Nov;31(1):11-24 PMID: 6186379
  17. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies.
    Cell. 1982 Sep;30(2):361-72 PMID: 6183000
  18. High frequencies of antigen-specific hybridomas: dependence on immunization parameters and prediction by spleen cell analysis.
    J Immunol Methods. 1980;32(3):297-304 PMID: 6153209
  19. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.
    Lab Invest. 1983 Apr;48(4):372-94 PMID: 6187996
  20. Monoclonal cytokeratin antibodies that distinguish simple from stratified squamous epithelia: characterization on human tissues.
    EMBO J. 1982;1(12):1641-7 PMID: 6202511
Article Info
Journal
Virchows Archiv. A, Pathological anatomy and histopathology
Abbr.
Virchows Arch A Pathol Anat Histopathol
ISSN
0174-7398
DOI
10.1007/BF00701324
Published
1985-00-00
Pages
1-12
Language
English
Region
Germany
NLM ID
8302198
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00701324
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