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PMID: 2410365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antibodies to O-antigen of lipopolysaccharide are protective against neonatal infection with Escherichia coli K1.

Infection and immunity ·Vol. 49 ·No. 2 ·1985-08-00 ·Pages 365-70

Pluschke G, Achtman M

Abstract

Monoclonal IgM specific for the O18 antigen conferred passive protection to 1-week-old rats against bacteremia and killing after oral challenge with O18:K1 Escherichia coli. Specific protection of the pups was also achieved by immunizing the pregnant rats with purified O18 lipopolysaccharide. We suppose that most human newborns that are colonized by potentially invasive K1 E. coli are protected by the transplacental transfer of anti-lipopolysaccharide immunoglobulin G, and we suggest that treatment with such antibodies might in the future be considered a therapeutic option. Rat serum from 1-week-old animals had only about one-third of the complement hemolytic activity of adult rat serum. This low level of hemolytic activity correlated with a relatively poor bactericidal activity in antibody-dependent and antibody-independent bactericidal in vitro assays. Monoclonal anti-O18 immunoglobulin M, although protective in vivo and bactericidal when added to adult rat serum, only poorly inhibited the multiplication of O18:K1 cells in serum from 1-week-old rats. This suggests that other elements of host defense besides complement participate in antibody-mediated in vivo protection.

MeSH Terms
Agglutination Tests Aging Animals Animals, Newborn Antibodies, Monoclonal/administration & dosage Antigens, Bacterial/immunology Complement System Proteins/immunology Escherichia coli/pathogenicity Escherichia coli Infections/immunology Hemolysis Hybridomas Immunization, Passive Immunoglobulin M/administration & dosage O Antigens Rats Rats, Inbred Strains Virulence
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Immunoglobulin M O Antigens Complement System Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pluschke G
Achtman M
References (28)
28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.2.365-370.1985
Published
1985-08-00
Pages
365-70
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262024
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.2.365-370.1985 PMC Full Text View journal details
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