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PMID: 241077 Published · ppublish English Journal Article

An octamer of histones in chromatin and free in solution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2626-30

Thomas JO, Kornberg RD

Abstract

Crosslinking with dimethyl suberimidate reveals a chain of histone octamers in chromatin. The octamer can be isolated free in solution at high ionic strength and pH. The identification of dimers formed by crosslinking reveals two or more contacts of each histone with others within the octamer. The molecular weight (110,000) and pattern of dissociation of the octamer are compatible with the composition (F2A1)2(F3)2(F2A2)2(F2B)2.

MeSH Terms
Animals Binding Sites Cell Nucleus/analysis Chromatin/analysis,ultrastructure Dimethyl Suberimidate Electrophoresis, Polyacrylamide Gel Histones Hydrogen-Ion Concentration Kinetics Liver/analysis Macromolecular Substances Molecular Weight Osmolar Concentration Protein Binding Rats
Chemicals
Chromatin Histones Macromolecular Substances Dimethyl Suberimidate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thomas J O
Kornberg R D
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2626
Published
1975-07-00
Pages
2626-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432822
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2626 PMC Full Text View journal details
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