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PMID: 2410929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regional distribution of calcium influx into bursting neurons detected with arsenazo III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5565-9

Graubard K, Ross WN

Abstract

Absorbance changes of the metallochromic indicator arsenazo III were used in conjunction with an array of 100 photodiodes to measure changes in intracellular calcium concentration at many positions simultaneously in identified neurons of the crab stomatogastric ganglion. When stimulated with intrasomatically injected current, several of these neurons showed calcium changes all over the cell, indicating that calcium channels were distributed widely in the neuropil and on the soma. When the membrane potential was allowed to oscillate without stimulation, absorbance oscillations were detected all over the neuropil but not in the soma. A comparison between the membrane potential recorded in the soma and the calcium signal in the neuropil shows that calcium entry followed the slow voltage oscillation with the peak calcium signal detected 50-150 msec after the end of the voltage plateau.

MeSH Terms
Animals Arsenazo III Azo Compounds Biological Transport, Active Brachyura Calcium/metabolism Ganglia/metabolism Ion Channels/metabolism Kinetics Neurons/metabolism,physiology Synaptic Transmission
Chemicals
Azo Compounds Ion Channels Arsenazo III Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Graubard K
Ross W N
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.16.5565
Published
1985-08-00
Pages
5565-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391163
Subset
IM
Grants
NINDS NIH HHS · R01 NS016295 · United States
NINDS NIH HHS · NS15697 · United States
NINDS NIH HHS · NS16295 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.16.5565 PMC Full Text View journal details
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