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PMID: 2413915 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Agonists Bay-K8644 and CGP-28392 open calcium channels reconstituted from skeletal muscle transverse tubules.

Biophysical journal ·Vol. 48 ·No. 2 ·1985-08-00 ·Pages 341-7

Affolter H, Coronado R

Abstract

The recently described calcium channel agonists Bay-K8644 and CGP-28392 have been used to induce long-term opening of calcium channels from purified rat muscle transverse tubules (t-tubules) incorporated into planar phospholipid bilayers. Agonist-open channels are selective for divalent cations (except Mg++), display voltage-dependent kinetics, and are blocked by the calcium channel antagonist, nitrendipine. The sensitivity to dihydropyridine agonists and antagonists indicate that a pool of t-tubule calcium channels remain functional after membrane fractionation and purification.

MeSH Terms
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester Animals Calcium/metabolism Calcium Channel Blockers/pharmacology Electric Conductivity Ion Channels/drug effects,physiology Kinetics Muscles/physiology Nifedipine/analogs & derivatives,pharmacology Pyridines/pharmacology
Chemicals
Calcium Channel Blockers Ion Channels Pyridines 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester CGP 28392 Nifedipine Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Affolter H
Coronado R
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22 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(85)83789-9
Published
1985-08-00
Pages
341-7
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1329327
Subset
IM
Grants
NIGMS NIH HHS · R01-GM32824 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(85)83789-9 PMC Full Text View journal details
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