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PMID: 2415976 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complexes of polyoma virus medium T antigen and cellular proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 23 ·1985-12-00 ·Pages 7952-4

Grussenmeyer T, Scheidtmann KH, Hutchinson MA, Eckhart W, Walter G

Abstract

Antibodies against synthetic peptides corresponding to the carboxyl-terminal six amino acids, Lys-Arg-Ser-Arg-His-Phe (KF), and an internal region, Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu (EE), of polyoma virus medium T antigen were used successively to purify medium T antigen by affinity chromatography. Medium T antigen from cell extracts was first bound to anti-KF antibodies and released from the immune complex with excess KF peptide; then it was bound to anti-EE antibodies and released with excess EE peptide. Two proteins, pp60c-src and a new protein of approximately equal to 61,000 Da (61-kDa protein), were copurified because they formed complexes with medium T antigen. The 61-kDa protein-medium T antigen complex was detected in extracts from wild-type-infected and transformed cells but not from cells infected with NG59 virus, which has a mutation in the medium T gene and is transformation defective. Instead, NG59 medium T antigen formed a complex with another cellular protein of approximately equal to 72,000 Da.

MeSH Terms
Animals Antigens, Viral, Tumor Immunosorbent Techniques Macromolecular Substances Mice Molecular Weight Polyomavirus/immunology,metabolism Protein Binding Proteins/metabolism Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Viral Proteins/metabolism
Chemicals
Antigens, Viral, Tumor Macromolecular Substances Proteins Proto-Oncogene Proteins Viral Proteins Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Grussenmeyer T
Scheidtmann K H
Hutchinson M A
Eckhart W
Walter G
References (18)
18 references, click to expand
  1. Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4666-70 PMID: 73183
  2. Phosphorylation sites in enolase and lactate dehydrogenase utilized by tyrosine protein kinases in vivo and in vitro.
    J Biol Chem. 1984 Jun 25;259(12):7835-41 PMID: 6330085
  3. Tumor antigens induced by nontransforming mutants of polyoma virus.
    Cell. 1978 Oct;15(2):485-96 PMID: 214241
  4. Identification of DNA sequence changes leading to loss of transforming ability in polyoma virus.
    J Biol Chem. 1980 Jan 10;255(1):230-5 PMID: 6243123
  5. Protein kinase activity associated with polyoma virus middle T antigen in vitro.
    Cell. 1979 Dec;18(4):915-24 PMID: 229972
  6. An activity phosphorylating tyrosine in polyoma T antigen immunoprecipitates.
    Cell. 1979 Dec;18(4):925-33 PMID: 229973
  7. Phosphorylation of polyoma T antigens.
    Cell. 1979 Dec;18(4):935-46 PMID: 229974
  8. Comparison of phosphorylation of two polyoma virus middle T antigens in vivo and in vitro.
    J Virol. 1981 Oct;40(1):184-96 PMID: 6169848
  9. Antibodies specific for the polyoma virus middle-size tumor antigen.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4882-6 PMID: 6272281
  10. Mutation causing premature termination of the polyoma virus medium T antigen blocks cell transformation.
    J Virol. 1982 Mar;41(3):1014-24 PMID: 6284958
  11. Carboxy terminus of polyoma middle-sized tumor antigen is required for attachment to membranes, associated protein kinase activities, and cell transformation.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3579-83 PMID: 6179082
  12. A one-step purification of membrane proteins using a high efficiency immunomatrix.
    J Biol Chem. 1982 Sep 25;257(18):10766-9 PMID: 6955305
  13. Purification of polyoma virus medium-size tumor antigen by immunoaffinity chromatography.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4025-9 PMID: 6287461
  14. Antibody to the nonapeptide Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu is specific for polyoma middle T antigen and inhibits in vitro kinase activity.
    J Biol Chem. 1982 Nov 10;257(21):12467-70 PMID: 6290466
  15. Polyoma virus middle T antigen: relationship to cell membranes and apparent lack of ATP-binding activity.
    Mol Cell Biol. 1982 Oct;2(10):1187-98 PMID: 6184609
  16. Polyoma virus transforming protein associates with the product of the c-src cellular gene.
    Nature. 1983 Jun 2-8;303(5916):435-9 PMID: 6304524
  17. G proteins and dual control of adenylate cyclase.
    Cell. 1984 Mar;36(3):577-9 PMID: 6321035
  18. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
    Immunochemistry. 1978 Jul;15(7):429-36 PMID: 30693
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.23.7952
Published
1985-12-00
Pages
7952-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390888
Subset
IM
Grants
NCI NIH HHS · CA36111 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.23.7952 PMC Full Text View journal details
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