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PMID: 2417224 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the cytokeratin endo A gene during early mouse embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8535-9

Duprey P, Morello D, Vasseur M, Babinet C, Condamine H, Brûlet P, Jacob F

Abstract

Expression of cytokeratin endo A has been analyzed during mouse blastocyst formation and embryonal carcinoma cell differentiation. To study the regulation of endo A expression, nuclease S1 mapping experiments have been performed on RNA extracted from two-cell to 7.5-day embryos. Low levels of endo A mRNA begin to be detectable in eight-cell embryos. The amount of this mRNA increases at the blastocyst stage, suggesting that endo A expression is regulated at the mRNA level during blastocyst formation. At this stage, in situ hybridization studies show that endo A mRNA is present in the trophectoderm but not in the inner cell mass. In 7.5-day embryos, endo A mRNAs are also detectable in the endoderm layer and in the amnion.

MeSH Terms
Animals Blastocyst/physiology,ultrastructure Embryonic Development Female Gene Expression Regulation Keratins/genetics,metabolism Mice/embryology Mice, Inbred Strains Morphogenesis Morula/physiology,ultrastructure Nucleic Acid Hybridization Pregnancy RNA, Messenger/genetics
Chemicals
RNA, Messenger Keratins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Duprey P
Morello D
Vasseur M
Babinet C
Condamine H
Brûlet P
Jacob F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8535
Published
1985-12-00
Pages
8535-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390951
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.24.8535 PMC Full Text View journal details
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