Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 88 ·No. 8 ·1994-09-00 ·Pages 1050-5

Timmerman GM, Frew TJ, Weeden NF, Miller AL, Goulden DS

Abstract

Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Timmerman G M

New Zealand Institute for Crop & Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.

Frew T J

Weeden N F

Miller A L

Goulden D S

References (9)

9 references, click to expand

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Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00220815

Published

1994-09-00

Pages

1050-5

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00220815 View journal details

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Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.).

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