Physical map of the COB region in mitochondrial DNA of Saccharomyces cerevisiae.

Current genetics ·Vol. 3 ·No. 1 ·1981-04-00 ·Pages 65-71

Grosch G, Schmelzer C, Mathews S

Abstract

A physical map of the COB region in mtDNA of yeast has been established. This region harbours a split gene coding for apocytochrome b. It includes restriction sites of seven endonucleases (EcoRI, HindII, HindIII, HaeIII, HpaII, AluI and BamHI). Various mtDNA sequences of this region, retained in a series of genetically characterized rho(-) clones have been allocated to this map. The combination of this physical map with a genetic map of the rho(-) clones revealed that 1) cob(-) mutational sites spread over 8,400 bp, 2) mutations in sequences coding for apocytochrome b map in five distinct segments which are separated by intervening sequences with minimum lengths from 350 to 1,900 bp.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Grosch G

Genetisches Institut der Universität München, Maria-Ward-Str. 1a, D-8000, München 19, Germany.

Schmelzer C

Mathews S

References (12)

12 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00419582

Published

1981-04-00

Pages

65-71

Language

English

Region

United States

NLM ID

8004904

External Links

PubMed source DOI: 10.1007/BF00419582 View journal details

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Physical map of the COB region in mitochondrial DNA of Saccharomyces cerevisiae.

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