31P magnetic resonance of tRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3482-5

Guéron M, Shulman RG

Abstract

We have observed well-resolved 31P resonances at 65 kG (109 MHz) in solutions of Escherichia coli tRNAGlu and yeast tRNAPhe. One resolved resonance, identified as the terminal phosphate, titrates with a pK = 6.35. Upon melting the yeast tRNAPhe in 0.1 M NaCl, without Mg++, the resolved peaks broaden and disappear in the vicinity of 35 degrees while the central cluster narrows drastically, shifts slightly, and loses its structure. Addition of Mg++ shifts one resolved peak and changes the shape of the cluster. The 31P lines are broader when observed at 65 kG than at 24 kG. The broadening is shown to come from chemical shift anisotropy which is estimated to be about 140 ppm.

MeSH Terms

Escherichia coli Hydrogen-Ion Concentration Magnesium/pharmacology Magnetic Resonance Spectroscopy Nucleic Acid Conformation/drug effects RNA, Bacterial RNA, Transfer Saccharomyces cerevisiae Temperature

Chemicals

RNA, Bacterial RNA, Transfer Magnesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Guéron M

Shulman R G

References (8)

8 references, click to expand

31P-NMR studies of tRNA.
FEBS Lett. 1974 Apr 15;41(1):40-2 PMID: 4212517
Peptide antibiotic-dinucleotide interactions. Nuclear magnetic resonance investigations of complex formation between actinomycin D and d-pGpC in aqueous solution.
Biochemistry. 1974 May 21;13(11):2388-95 PMID: 4831638
Conformational differences between the biologically active and inactive forms of a transfer ribonucleic acid.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1684-91 PMID: 5231404
Frequency dependence of 31P NMR linewidths in sonicated phospholipid vesicles: effects of chemical shift anisotropy.
FEBS Lett. 1974 Sep 15;46(1):55-8 PMID: 4425071
High resolution NMR study of the melting of yeast tRNA Phe.
Biochem Biophys Res Commun. 1973 Dec 10;55(3):953-60 PMID: 4586623
31P magnetic resonance of purified tRNA.
FEBS Lett. 1971 Dec 15;19(3):264-266 PMID: 11946227
NUCLEAR MAGNETIC RESONANCE OF PHOSPHORUS IN DEOXYTHYMIDINE POLYNUCLEOTIDES.
Nature. 1964 Jul 18;203:301-2 PMID: 14201769
The conformational transitions in yeast tRNAPhe as studied with tRNAPhe fragments.
Eur J Biochem. 1973 Jul 2;36(1):76-88 PMID: 4581822

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.9.3482

Published

1975-09-00

Pages

3482-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433018

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.9.3482 PMC Full Text View journal details

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31P magnetic resonance of tRNA.

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