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PMID: 2421256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and nucleotide sequence determination of a potential primer tRNA for reverse transcription of a Drosophila retrotransposon, 297.

Nucleic acids research ·Vol. 14 ·No. 7 ·1986-04-11 ·Pages 3031-43

Inouye S, Saigo K, Yamada K, Kuchino Y

Abstract

Drosophila retrotransposons have been shown to have genes for enzymes similar to the reverse transcriptase of retroviruse. They may possibly be involved in genome replication on translocation (15). Identification was made of a primer tRNA for the putative reverse transcription of a Drosophila retrotransposon, 297, and its genomic counterparts using a homology to the putative primer binding site of 297. Our nucleotide sequence analysis indicated a species of Drosophila serine tRNA to have two distinct properties similar to those characteristic of retroviral primer tRNA: its 3'terminal 18 nucleotides are exactly complementary to the putative primer binding site of 297 and its 19th base from the 3' end is modified. These results appear to support the notion stated above and suggest this serine tRNA to be the most likely candidate for a potential primer tRNA of 297.

MeSH Terms
Animals Base Sequence DNA Transposable Elements Drosophila melanogaster Nucleic Acid Conformation RNA, Transfer/analysis RNA-Directed DNA Polymerase/metabolism Transcription, Genetic
Chemicals
DNA Transposable Elements RNA, Transfer RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Inouye S
Saigo K
Yamada K
Kuchino Y
References (32)
32 references, click to expand
  1. Origin of retroviruses from cellular moveable genetic elements.
    Cell. 1980 Oct;21(3):599-600 PMID: 6254661
  2. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  3. Extrachromosomal circular copies of the eukaryotic transposable element copia in cultured Drosophila cells.
    Nature. 1981 Aug 13;292(5824):591-5 PMID: 6265802
  4. Copia-like transposable elements in the Drosophila genome.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:619-28 PMID: 6266755
  5. Mobile dispersed genetic elements and other middle repetitive DNA sequences in the genomes of Drosophila and mouse: transcription and biological significance.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:641-54 PMID: 6266756
  6. General properties of mobile dispersed genetic elements in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:655-65 PMID: 6266757
  7. Structural analogies among avian retroviral DNAs and transposable elements.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:739-46 PMID: 6266766
  8. The organization of Drosophila melanogaster histone genes.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:815-27 PMID: 6266769
  9. Mobile dispersed genetic element MDG1 of Drosophila melanogaster: nucleotide sequence of long terminal repeats.
    Nucleic Acids Res. 1981 Jul 24;9(14):3451-64 PMID: 6269083
  10. Drosophila genome organization: conserved and dynamic aspects.
    Annu Rev Genet. 1981;15:219-64 PMID: 6802066
  11. Nucleotide sequence of terminal repeats of 412 transposable elements of Drosophila melanogaster. A similarity to proviral long terminal repeats and its implications for the mechanism of transposition.
    J Mol Biol. 1981 Dec 25;153(4):897-915 PMID: 6283088
  12. Structure of a replication intermediate in the synthesis of Rous sarcoma virus DNA in vivo.
    J Virol. 1982 Apr;42(1):337-41 PMID: 6283154
  13. Transcription initiation of eucaryotic transfer RNA genes.
    Cell. 1982 May;29(1):3-5 PMID: 7049399
  14. Insertion of a movable genetic element, 297, into the T-A-T-A box for the H3 histone gene in Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1982 Jul;79(13):4143-7 PMID: 6287469
  15. B104, a new dispersed repeated gene family in Drosophila melanogaster and its analogies with retroviruses.
    J Mol Biol. 1982 May 25;157(3):435-51 PMID: 6181263
  16. Nucleotide sequences of rat liver serine-tRNA. 3. The partial enzymatic of serine-tRNA and derivation of its total primary structure.
    Eur J Biochem. 1971 Jul 29;21(2):249-57 PMID: 5105577
  17. A transposable element that splits the promoter region inactivates a Drosophila cuticle protein gene.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7430-4 PMID: 6296833
  18. Retrovirus-like particles containing RNA homologous to the transposable element copia in Drosophila melanogaster.
    Nature. 1983 Mar 10;302(5904):119-24 PMID: 6186922
  19. Close relationship between the long terminal repeats of avian leukosis-sarcoma virus and copia-like movable genetic elements of Drosophila.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3193-7 PMID: 6304696
  20. The origin of extrachromosomal circular copia elements.
    Cell. 1983 Sep;34(2):415-9 PMID: 6616617
  21. Mutations in Box B of the promoter of a eucaryotic tRNAPro gene affect rate of transcription, processing, and stability of the transcripts.
    Cell. 1984 Jan;36(1):179-87 PMID: 6559106
  22. Interactions between avian myeloblastosis reverse transcriptase and tRNATrp. Mapping of complexed tRNA with chemicals and nucleases.
    Nucleic Acids Res. 1984 Mar 12;12(5):2259-71 PMID: 6200830
  23. Structural organization of transposable element mdg4 from Drosophila melanogaster and a nucleotide sequence of its long terminal repeats.
    Nucleic Acids Res. 1984 Apr 25;12(8):3707-23 PMID: 6328434
  24. Sequence-specific insertion of the Drosophila transposable genetic element 17.6.
    Nature. 1984 Jul 26-Aug 1;310(5975):332-3 PMID: 6087153
  25. Role of reverse transcription in the generation of extrachromosomal copia mobile genetic elements.
    Nature. 1984 Aug 9-15;310(5977):514-6 PMID: 6205279
  26. Long terminal repeat nucleotide sequence and specific insertion of the gypsy transposon.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4462-4 PMID: 6087324
  27. Reverse transcription of Drosophila mobile dispersed genetic element RNAs: detection of intermediate forms.
    Nucleic Acids Res. 1984 Oct 11;12(19):7533-48 PMID: 6208533
  28. Identification of the coding sequence for a reverse transcriptase-like enzyme in a transposable genetic element in Drosophila melanogaster.
    Nature. 1984 Dec 13-19;312(5995):659-61 PMID: 6209583
  29. Ty elements transpose through an RNA intermediate.
    Cell. 1985 Mar;40(3):491-500 PMID: 2982495
  30. Steady-state kinetic analysis of transcription of cloned tRNASer genes from Drosophila melanogaster.
    Eur J Biochem. 1985 Apr 15;148(2):305-13 PMID: 3921375
  31. Close structural resemblance between putative polymerase of a Drosophila transposable genetic element 17.6 and pol gene product of Moloney murine leukaemia virus.
    EMBO J. 1985 May;4(5):1267-72 PMID: 2408886
  32. The nucleotide sequences of copia and copia-related RNA in Drosophila virus-like particles.
    Nature. 1985 Jun 27-Jul 3;315(6022):773-6 PMID: 2409449
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.7.3031
Published
1986-04-11
Pages
3031-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339719
Subset
IM
Databases
GENBANK
X03730, X03731, X03732, X03815
External Links
PubMed source DOI: 10.1093/nar/14.7.3031 PMC Full Text View journal details
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