Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 79 ·No. 2 ·1990-02-00 ·Pages 177-81

Schmidt T, Junghans H, Metzlaff M

Abstract

Beta procumbens-specific DNA probes have been constructed by cloning digested total DNA in E. coli and screening the resulting recombinant plasmids in dot blot hybridizations with labelled B. procumbens and B. vulgaris DNA. Four clones (pTS1-4) have been analyzed in detail determining their degree of specificity and DNA sequence. Two clones (pTS1 and pTS2) with the highest degree of B. procumbens specificity were adapted for the squash dot hybridization with the aim of screening large numbers of individual hybrid plants (B. vulgaris x B. procumbens) carrying an alien B. procumbens chromosome (2n = 19). These addition lines carry in some cases B. procumbens resistance genes to the beet cyst nematode (Heterodea schachtii Schm.).

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schmidt T

Department of Genetics, Biosciences Section, Martin-Luther-University, Halle-Wittenberg, German Democratic Republic.

Junghans H

Metzlaff M

References (12)

12 references, click to expand

Genome size and the proportion of repeated nucleotide sequence DNA in plants.
Biochem Genet. 1974 Oct;12(4):257-69 PMID: 4441361
Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test.
Theor Appl Genet. 1988 Nov;76(5):728-32 PMID: 24232351
A rapid screening technique for the detection of repeated DNA sequences in plant tissues.
Theor Appl Genet. 1985 May;69(3):329-33 PMID: 24253829
Organization and evolution of a higher plant alphoid-like satellite DNA sequence.
J Mol Biol. 1986 Feb 20;187(4):495-507 PMID: 3012090
Isolation and characterisation of a tandemly repeated DNA sequence from the crucifer Sisymbrium irio.
Indian J Biochem Biophys. 1988 Dec;25(6):483-7 PMID: 3255663
A molecular description of telometic heterochromatin in secale species.
Cell. 1980 Feb;19(2):545-60 PMID: 6244112
Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.
Curr Genet. 1984 Sep;8(7):499-506 PMID: 24177950
Monosomic additions in beet (Beta vulgaris) carrying extra chromosomes of Beta procumbens : 1. Identification of the alien chromosomes with the help of isozyme markers.
Theor Appl Genet. 1988 Oct;76(4):577-86 PMID: 24232280
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Wheat specific repetitive DNA sequences - construction and characterization of four different genomic clones.
Theor Appl Genet. 1986 Mar;72(2):207-10 PMID: 24247835
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356

Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00225948

Published

1990-02-00

Pages

177-81

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00225948 View journal details

Analysis Services

Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines.

Contact

WeChat Official Account