Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons.

Planta ·Vol. 166 ·No. 2 ·1985-10-00 ·Pages 151-5

Burgess N, Beakes GW, Thomas DR

Abstract

A method is described for separating mitochondria from microbodies in cotyledon preparations of Pisum sativum L. cv. Alaska. Pure and intact mitochondria were obtained on a continuous: discontinuous sucrose density gradient as shown by marke-enzyme assay and electron microscopy. Manipulation of sucrose-gradient construction to widen the distance between organelles provided a quick method for the separation of the mitochondria from the microbodies. The shorter time of exposure of mitochondria to centrifugation and osmotic stress produces mitochondria free of contamination.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Burgess N

Department of Plant Biology, Newcastle University, NE1 7RU, Newcastle upon Tyne, UK.

Beakes G W

Thomas D R

References (9)

9 references, click to expand

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Article Info

Journal

Planta

Abbr.

Planta

ISSN

0032-0935

DOI

10.1007/BF00397341

Published

1985-10-00

Pages

151-5

Language

English

Region

Germany

NLM ID

1250576

External Links

PubMed source DOI: 10.1007/BF00397341 View journal details

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Separation of mitochondria from microbodies of Pisum sativum (L. cv. Alaska) cotyledons.

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