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PMID: 2425781 Published · ppublish English Comparative Study Journal Article

Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies.

Archives of virology ·Vol. 90 ·No. 1-2 ·1986-00-00 ·Pages 97-110

Abenes G, Kida H, Yanagawa R

Abstract

Twelve monoclonal antibodies to the F protein of a velogenic strain of Newcastle disease virus (NDV) were established. Each of these antibodies inhibited virus-induced plaque formation in BHK-21 cells. Seven antibodies neutralized viral infectivity in eggs; thus, antigenic variants could be selected with these antibodies and used for antigenic mapping. Based on the reactivity of the antigenic variants with the antibodies used in selection, 4 distinct antigenic sites (I-IV) were defined on the F protein molecule. In competitive binding assay, sites II and III were found to be spatially close to each other. Each antibody to sites I, II and III inhibited both virus-induced hemolysis of chicken erythrocytes and syncytium formation of BHK-21 cells. On the other hand, some of the antibodies to site IV selectively inhibited either hemolysis or cell fusion. This finding may indicate that the fusion of the viral envelope with erythrocytes and host cell membrane is modulated through different ways. Comparative analysis of different NDV strains using monoclonal antibodies to each of the different antigenic sites showed that the antigenicity of the F protein is highly conserved.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibodies, Viral/immunology Antigens, Viral/immunology Cell Fusion Cell Line Chickens Cricetinae Epitopes/immunology Erythrocytes Kidney Mesocricetus Newcastle disease virus/immunology Paramyxoviridae/immunology Viral Envelope Proteins/immunology Viral Fusion Proteins
Chemicals
Antibodies, Monoclonal Antibodies, Viral Antigens, Viral Epitopes Viral Envelope Proteins Viral Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Abenes G
Kida H
Yanagawa R
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43 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01314148
Published
1986-00-00
Pages
97-110
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01314148 View journal details
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