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PMID: 2426471 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of reverse transcriptase activity of human T-lymphotropic virus type III (HTLV-III/LAV) in Escherichia coli.

Journal of virology ·Vol. 59 ·No. 3 ·1986-09-00 ·Pages 743-5

Tanese N, Sodroski J, Haseltine WA, Goff SP

Abstract

The pol gene from a biologically active clone of the human T-cell lymphotropic virus type III provirus was inserted into a bacterial expression vector. The resulting gene fusion induced the formation of active reverse transcriptase that could be readily detected in extracts of bacterial cells. The activity exhibited the template and divalent cation requirements of the authentic enzyme. These constructs will be useful for safe and rapid analysis of potential inhibitors of this important enzyme.

MeSH Terms
DNA, Recombinant Deltaretrovirus/enzymology,genetics Escherichia coli/enzymology,genetics Genes, Viral Magnesium/pharmacology Manganese/pharmacology Oligodeoxyribonucleotides/metabolism Poly A/metabolism Poly C/metabolism RNA-Directed DNA Polymerase/biosynthesis,genetics,metabolism
Chemicals
DNA, Recombinant Oligodeoxyribonucleotides poly (rC).oligo (dG) Poly A Poly C Manganese poly(rA).oligo(dT) RNA-Directed DNA Polymerase Magnesium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tanese N
Sodroski J
Haseltine W A
Goff S P
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17 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.59.3.743-745.1986
Published
1986-09-00
Pages
743-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253253
Subset
IM
Grants
NCI NIH HHS · CA30488 · United States
NCI NIH HHS · CA40658 · United States
External Links
PubMed source DOI: 10.1128/JVI.59.3.743-745.1986 PMC Full Text View journal details
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